6.1 Cellular Control Flashcards
What is a gene mutation?
Change to DNA
How might they occur?
- randomly during DNA replication
- mutagenic agents such as UV light, tobacco, gamma rays etc
What is the difference between mutations in mitotic division and meiosis?
Mitotic -> cannot be passed, form cancerous tumours
Meiosis -> can be passed, in gamete formation
What is a silent point mutation?
- substitution of a base in a triplet but still coding for the same amino acid due to DEGENERACY
What is a missense point mutation?
- change to triplet causing DIFF amino acid sequence
- alteration of primary, etc, tertiary structure of protein -> might affect function
What is an example of a missense point mutation?
- sickle cell anaemia
What is a nonsense point mutation? What is an example?
- altering of a base triplet, so it becomes a stop codon
- truncated protein that will not function
- Duchenne muscular dystrophy
What is an indel mutation? What is an example?
- nucleotide bases, NOT IN MULTIPLES OF 3, get inserted or deleted
- all subsequent triplets are altered due to overlapping nature of code + FRAMESHIFT
- primary, etc, tertiary structure altered.
- some forms of thalassemia
What is the significance of an expanding triple nucleotide repeat? What is an example?
- number of repeats increases at meiosis and again from generation to generation
- Huntingtons: if CAG repeats go above a certain critical number
If glucose is absent and lactose is present, what occurs?
- induces production of lactose permease (allows it to enter) and B-galactosidase (hydrolyses to galactose and glucose)
What is lac0?
operator region
What is lacZ?
codes for B-galactosidase
What is lacY?
codes for lactose permease
What is P?
promotor region where RNA polymerase binds to begin transcription
What is I?
when expressed, LacI repressor protein is coded for which binds to operator -> prevents RNA polymerase from binding and prevents transcription
What process occurs when lactose is present?
regulatory gene switches on and transcription of mRNA and then translation of repressor protein occurs
this binds to lactose, forming an inducer-repressor complex so it cannot bind to lac0 anymore
lacZ and lacY can therefore be transcribed into mRNA and this can be translated into the two required proteins to use lactase.
What are transcription factors?
proteins/short non coding mRNA that bind to their specific promoter region, aid/inhibit RNA polymerase attachment, and activate/suppress transcription
What are transcription factors involved with in eukaryotic cells?
- cell cycle (tumour suppressor + proto-oncogenes use them)
What happens in post-transcriptional modification/gene regulation? What happens to the introns?
- full transcription unit including exons (coding) AND introns (non-coding) is transcribed into primary mRNA.
- splicing occurs to form the mature mRNA (introns removed, remaining exons joined together)
- some may become short lengths of non-coding RNA involved in gene regulation
A length of DNA with introns and exons can…..
encode more than one protein, depending on the splicing (alternative splicing)
What occurs in post-translational modification/gene regulation?
- activation of proteins via PHOSPHORYLATION
1) hormone/signal binds to receptor
2) G protein activated
3) adenyl cyclase activated
4) catalyses cAMP from ATP
5) cAMP activates PKA
6) phosphorylation of proteins occurs, activating many enzymes. uses ATP
7) using ATP, many also phosphorylate another protein such as CREB
8) CREB enters nucleus and acts as a transcription factor
What is the homeobox gene sequence?
sequence of 180 base pairs in animals, fungi and plants
What does the homeobox gene sequence encode for?
60 amino acid sequence called homeodomain sequence
What is the structure and function of the homeodomain sequence?
can regulate transcription of adjacent genes involved in development
H-T-H (2 a-helices and one turn)
part of sequence recognises TAAT on enhancer region of gene to be transcribed