6. Plant Nutrition Flashcards
Photosynthesis definition
the process by which plants manufacture carbohydrates from raw materials using energy from light
What are the raw materials for photosynthesis?
carbon dioxide and water
What is the waste product of photosynthesis?
Oxygen
Word equation for photosynthesis
light
carbon dioxide + water –> glucose + oxygen
chlorophyll
Where is water for photosynthesis taken from?
Taken up by the roots and transported through the xylem to the leaves
Where is carbon dioxide for photosynthesis taken from?
Diffuses into the leaf through the stomata
What is glucose in plants used for?
Used to make substances needed by the plant
- used in respiration as a source of energy
What happens to oxygen produced by photosynthesis?
- Diffuses out of the leaf through the stomata
- used in respiration
What is the balanced chemical equation for photosynthesis?
6CO2 + 6H2O —> C6H12O6 + 6O2
What happens to the light energy during photosynthesis?
The light energy is converted into chemical energy in the bonds holding the atoms in the glucose molecules together
What substances (that the plant needs) can be made from glucose?
They can also convert it into:
- starch for storage
- into lipids for an energy source in seeds
- into cellulose to make cell walls
- into amino acids (used to make proteins) when combined with nitrogen and other mineral ions absorbed by roots
Why do plants convert glucose into starch, rather than keeping it as glucose?
It is insoluble, meaning it won’t affect osmosis in cells
What can glucose be converted into to be transported around the plant?
converted into sucrose
Why can leaves not be tested for glucose?
Although plants make glucose in photosynthesis, leaves cannot be tested for its presence as the glucose is quickly used, converted into other substances and transported or stored as starch.
Where is starch stored?
Starch is stored in chloroplasts where photosynthesis occurs
What test can be a reliable indicator of which parts of the leaf are photosynthesising?
testing a leaf for starch
Procedure for testing leaves for starch
- A leaf is dropped in boiling water to kill and break down the cell walls
- The leaf is left for 5-10 minutes in hot ethanol in a boiling tube. This removes the chlorophyll so colour changes from iodine can be seen more clearly
- The leaf is dipped in boiling water to soften it
- The leaf is spread out on a white tile and covered with iodine solution
- In a green leaf, the entire leaf will turn blue-black as photosynthesis is occurring in all areas of the leaf
How does the results for testing leaves for starch change if the leaf is variegated?
What can this test also be used to test?
- This method can also be used to test whether chlorophyll is needed for photosynthesis by using a variegated leaf (one that is partially green and partially white)
- The white areas of the leaf contain no chlorophyll and when the leaf is tested only the areas that contain chlorophyll stain blue-black
- The areas that had no chlorophyll remain orange-brown as no photosynthesis is occurring here and so no starch is stored
What safety precautions must be taken into account when testing a leaf for starch?
Care must be taken when carrying out this practical as ethanol is extremely flammable, so at that stage of the experiment the Bunsen burner should be turned off.
What is the safest way to heat ethanol when testing a leaf for starch?
The safest way to heat the ethanol is in an electric water bath rather than using a beaker over a Bunsen burner with an open flame
How can the experiment for testing leaves for starch be altered to show the need for light in photosynthesis?
- Before starting the experiment the plant needs to be destarched by placing in a dark cupboard for 24 hours
- This ensures that any starch already present in the leaves will be used up and will not affect the results of the experiment
- Following destarching, a leaf of the plant can be partially covered with aluminium foil and the plant placed in sunlight for a day
- The leaf can then be removed and tested for starch using iodine
- The area of the leaf that was covered with aluminium foil will remain orange-brown as it did not receive any sunlight and could not photosynthesise, while the area exposed to sunlight will turn blue-black
- This proves that light is necessary for photosynthesis and the production of starch
Procedure for The Need for Carbon Dioxide in Photosynthesis
- Destarch a plant
- Tie a clear bag containing sodium hydroxide, which will absorb carbon dioxide from the surrounding air, around one leaf
- Tie a clear bag containing water (control experiment), which will not absorb carbon dioxide from the surrounding air, around another leaf
- Place the plant in bright light for several hours.
- Test both leaves for starch using iodine
- The leaf from the bag containing sodium hydroxide will remain orange-brown as it could not photosynthesise due to lack of carbon dioxide
- The leaf from the control bag containing water should turn blue-black as it had all necessary requirements for photosynthesis
What plants are usually used to investigate the rate of photosynthesis?
The plants usually used are Elodea or Camboba – types of pondweed
How is the rate of photosynthesis measured?
- As photosynthesis occurs, oxygen gas produced is released
- As the plant is in water, the oxygen released can be seen as bubbles leaving the cut end of the pondweed
- The number of bubbles produced over a minute can be counted to record the rate
- The more bubbles produced per minute, the faster the rate of photosynthesis
What can be a more accurate way of measuring the rate of photosynthesis rather than counting the number of bubbles produced?
- A more accurate version of this experiment is to collect the oxygen released in a test tube inverted over the top of the pondweed over a longer period of time and then measure the volume of oxygen collected
What factors can be investigated to see how they influence the rate of photosynthesis?
- Investigating the effect of changing light intensity, by moving a lamp different distances away from the beaker containing the pondweed
- Investigating the effect of changing temperature, by changing the temperature of the water in the beaker
- Investigating the effect of changing carbon dioxide concentration, by dissolving different amounts of sodium hydrogen carbonate in the water in the beaker
What precautions must be taken when investigating the rate of photosynthesis?
Care must be taken when investigating a condition to keep all other variables constant in order to ensure a fair test
Why does a glass tank need to be used when when investigating changing light intensity?
When investigating changing light intensity, a glass tank should be placed in between the lamp and the beaker to absorb heat from the lamp and so avoid changing the temperature of the water as well as the light intensity
What is the rate of photosynthesis limited by?
limited by whatever factor is the lowest at that time
What factors affect the rate of photosynthesis?
- light intensity
- carbon dioxide concentration
- temperature
Limiting factor definition
something present in the environment in such short supply that it restricts life processes
Why is water not considered a limiting factor?
Although water is necessary for photosynthesis, it is not considered a limiting factor as the amount needed is relatively small compared to the amount of water transpired from a plant so there is hardly ever a situation where there is not enough water for photosynthesis
How does temperature affect the rate of photosynthesis?
- As temperature increases the rate of photosynthesis increases as the reaction is controlled by enzymes
- However, as the reaction is controlled by enzymes, this trend only continues up to a certain temperature beyond which the enzymes begin to denature and the rate of reaction decreases
How does light intensity affect the rate of photosynthesis?
- The more light a plant receives, the faster the rate of photosynthesis
- This trend will continue until some other factor required for photosynthesis prevents the rate from increasing further because it is now in short supply