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BN (CT) > 6: blood collection tubes > Flashcards

6: blood collection tubes Flashcards

0
Q

if the tube has a barcode what machine is it to be used in?

A

The idex machine

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1
Q
  1. lavender or purple tops (LTT , PTT) contain?

2. and are used for what?

A
  1. anticoagulant EDTA

2. CBC and BUN

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2
Q

LTT/PTT

what is the fluid portion if spun called?

A

plasma

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3
Q

How does the anticoagulant EDTA work?

A

binds with calcium to prevent coagulation

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4
Q

List 3 advantages of LTT/PTT tubes?

A
  1. causes least amount of cell distortion/ destruction
  2. best preservation of cells
  3. fairly stable for 4-6 hrs
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5
Q

list 3 disadvantages of LTT/PTT tubes?

A
  1. after 6 hrs, WBC begin to break down and disintegrate
  2. after 4 hrs, platelets swell and clump
  3. incorrect blood to EDTA ratio causes cells to shrink
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6
Q

special considerations for LTT/PTT tubes:

  1. what should you do if not able to run tests immediately? Why?
  2. what is the benefit of rotating the tubes gently and thoroughly?
A
  1. refrigerate immediately. It minimizes cell damage

2. prevents clotting and hemolysis

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7
Q

what do green top tubes (GTT) contain?

A

heparin which prevents conversion of prothrombin to thrombin

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8
Q

what are the indicated uses of GTT tubes?

A

use in blood gas determination

example: CO2 and O2 levels

GREEN for GAS

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9
Q

what is the fluid portion if spun in GTT tubes called?

A

plasma

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10
Q

what are 3 advantages of GTT tubes?

A
  1. maintains size and shape of RBC even in poor blood sample
  2. best for blood gas reading, hormone, and drug levels in blood
  3. used for reptile hematologic studies
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11
Q

what are 3 disadvantages of GTT tubes?

A
  1. causes hazy staining with routine type differential stains
  2. WBC may clump
  3. expensive
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12
Q

if the GTT contains Lithium what will be its indicated use?

A

used for blood chemistries and in clinics that use the VET Test blood chemistry machine

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13
Q

What do blue top tubes (BTT) contain?

A

anticoagulant Sodium Citrate

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14
Q

in BTT how does the Sodium Citrate work?

A

binds with Calcium to prevent coagulation

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15
Q

what is the indicated use for BTT tubes?

A

for coagulation studies

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16
Q

what is the fluid portion in BTT if spun called?

A

citrated plasma

17
Q

What are 2 advantages of using BTT tubes?

A
  1. used for most coagulation studies needing anticoagulated blood
  2. non-toxic and can be used for blood transfusions
18
Q

what are 2 disadvantages of using BTT tubes?

A
  1. shrinks cells

2. tube must be filled. ratio of blood to anticoagulant is crucial

19
Q

what do grey top tubes (GTT) contain?

A

anticoagulant Oxylate (NH4 or K)

20
Q

in grey top tubes how does oxylate work?

A

binds with calcium to prevent coagulation

21
Q

what is the indicated use for grey top tubes

A

blood glucose levels

GREY for GLUCOSE

22
Q

what is the fluid portion in grey top tubes called?

A

plasma

23
Q

what is the advantage to using grey top tubes

A

maintains cell wall integrity eliminating movement of glucose from plasma into the cell

24
Q

what are 4 disadvantages of grey top tubes?

A
  1. K oxylate shrinks cells (K is smaller than NH4 so it shrinks)
  2. NH4 oxylate swells cells (NH4 is bigger than K so it swells)
  3. cannot be used for BUN due to NH4
  4. Oxylate crystals seen on differential, so not able to use for CBC
25
Q

What do red top tubes (RTT) contain?

A

no anticoagulant so blood will clot

some can have a gel substance in them

and some can have a white powder in them

26
Q

since the blood clots in RTT and settles to the bottom, what is the fluid portion called?

A

Serum

27
Q

what is the indicated use for RTT tubes?

A

blood chemistry studies

28
Q

since RTT blood glucose studies are poor, what should you do?

A

you should draw up a Grey top tube as well

29
Q

if the RTT has a gel like substance in it, what is the tube called and what is the benefit of this?

A

Serum separator tube

when spun the gel will act as a barrier not letting the clotted blood remix with the Serum.

it can be used for glucose studies

30
Q

if a RTT does not have gel or white powder what can it be used for?

A

blood or urine

31
Q

what will a tiger top tube contain?

A

gel substance, no anticoagulant

32
Q

if a grey top tube has a blue liquid then what does it not contain?

A

oxylate

33
Q

if a green top tube has heparin in it what will the tube be made of?

A

glass

34
Q

if a green top tube has lithium in it what will the tube be made of?

A

plastic

35
Q

what are the 4 plasma colors

A

clear
red
yellow
white

36
Q

what is the term and cause of a red plasma color

A

red term - hemolytic (ruptured blood cells)

red cause- multiple errors

37
Q

what is the term and cause of a yellow plasma color

A

yellow term - Icteric

yellow cause - Liver failure

38
Q

what is the term and cause of a white plasma color

A

white term - Lipemic

white cause - Fat, diabetes, hypothyroidism

39
Q

if a plasma color is orange what is the term

A

red- hemolytic

yellow - Icteric

40
Q

if the plasma color is pink what is the term

A

red - hemolytic

white - lipemic

41
Q

if the plasma color is a cream color what is the term

A

yellow - Icteric

white - Lipemic

BN (CT) (4 decks)

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