5. Energy transfers in and between organisms Flashcards
Where does the light-dependent reaction occur?
In the thylakoids of chloroplasts
Where does the light-independent reaction occur?
In the stroma of chloroplasts
Explain the role of light in photoionisation.
Chlorophyll molecules absorb energy from photons of light. This ‘excites’ 2 electrons, causing them to be released from the chlorophyll.
Name the 2 main stages involved in ATP production in the light-dependent reaction.
Electron transfer chain
Chemiosmosis
What happens in the electron transfer chain (ETC)?
Electrons released from chlorophyll move down a series of carrier proteins embedded in the thylakoid membrane and undergo a series of redox reactions, which release energy.
How is a proton concentration gradient established during chemiosmosis?
Some energy released from the electron transfer chain is coupled to the active transport of H+ ions (protons) from the stroma into the thylakoid space.
How does chemiosmosis produce ATP in the light-dependent stage?
H+ ions move down their concentration gradient from the thylakoid space into the stroma via the channel protein ATP synthase.
ATP synthase catalyses ADP + Pi → ATP
Explain the role of light in photolysis.
Light energy splits molecule of water
2H20 → 4H+ + 4e- + O2
What happens to the products of the photolysis of water?
H+ ions: move out of thylakoid space via ATP synthase and are used to reduce the coenzyme NADP.
e-: replace electrons lost from chlorophyll.
O2: used for respiration or diffuses out of leaf as waste gas.
How and where is reduced NADP produced in the light-dependent reaction?
NADP + 2H+ + 2e- → reduced NADP
Catalysed by dehydrogenase enzymes.
Stroma of chloroplasts.
Where do the H+ ions and electrons used to reduce NADP come from?
H+ ions: photolysis of water
Electrons: NADP acts as the final electron acceptor of the electron transfer chain.
Name the 3 main stages in the Calvin cycle.
Carbon fixation
Reduction
Regeneration
What happens during carbon fixation?
Reactions between CO2 and RuBP catalysed by rubisco.
Forms unstable 6C intermediate that breaks down into 2x GP.
What happens during reduction (in the Calvin cycle)?
2x GP are reduced to 2x TP
Requires 2x reduced NADP and 2x ATP
Forms 2x NADP and 2x ATP
How does the light-independent reaction result in the production of useful organic substances?
1C leaves the cycle (i.e. some of the TP is converted into useful organic molecules).
What happens during regeneration (in the Calvin cycle)?
After 1C leaves the cycle, the 5C compound RuP forms.
RuBP is regenerated from RuP using 1x ATP.
Forms 1x ADP.
State the roles of ATP and (reduced) NADP in the light-independent reaction.
ATP: reduction of GP to TP and provides phosphate group to convert RuP into RuBP.
(Reduced) NADP: coenzyme transports electrons needed for reduction of GP to TP.
State the number of carbon atoms in RuBP, GP and TP.
RuBP: 5
GP: 3
TP: 3
Describe the structure of a chloroplasts.
Usually disc-shaped.
Double membrane (envelope).
Thylakoids: flattened discs stack to form grana.
Intergranal lamellae: tubular extensions attach thylakoids in adjacent grana.
Stroma: fluid-filled matrix
How does the structure of the chloroplast maximise the rate of the light-dependent reaction?
ATP synthase channels within granal membrane.
Large surface area of thylakoid membrane for ETC.
Photosystems position chlorophyll to enable maximum absorption of light.
How does the structure of the chloroplast maximise the rate of the light-independent reaction?
Own DNA and ribosomes for synthesis of enzymes e.g. rubisco.
Concentration of enzymes and substrates in stroma is high.
Define ‘limiting factor’.
Factor that determines maximum rate of a reaction, even if other factors change to become more favourable.
Name 4 environmental factors that can limit the rate of photosynthesis.
Light intensity (LDR)
CO2 levels (LIR)
Temperature (enzyme-controlled steps)
Mineral/magnesium levels (maintain normal function of chlorophyll).
Outline some common agricultural practices used to overcome the effect of limiting factors in photosynthesis.
Artificial light, especially at night.
Artificial heating
Addition of CO2 to greenhouse atmosphere.
Why do farmers try to overcome the effect of limiting factors?
To increase yield.
Additional cost must be balanced with yield to ensure maximum profit.
Suggest how a student could investigate the effect of a name variable on the rate of photosynthesis.
dependent variable: rate of O2 production/CO2 consumption
Use a potometer.
Place balls of calcium alginate containing green algae in hydrogencarbonate indicator (colour change orange → magenta as CO2 is consumed and pH increases).
State the purpose and principle of paper chromatography.
Molecules in a mixture are separated based on their relative attraction to the mobile phase (running solvent) vs the stationary phase (chromatography paper).
Outline a method for extracting photosynthetic pigments.
Use a pestle and mortar to grind a leaf with an extraction solvent e.g. propanone.
Outline how paper chromatography can be used to separate photosynthetic pigments.
Use a capillary tube to spot pigment extract onto pencil ‘start line’ (origin) 1cm above bottom of paper.
Place chromatography paper in solvent (origin should be above solvent line).
Allow solvent to run until it almost touches the other end of the paper. Pigments move different distances.
What are Rf values and how can they be calculated?
Ratios that allow comparison of how far molecules have moved in chromatograms.
Rf = distance between origin and centre of pigment spot / distance between origin and solvent front
Name the 4 main stages in aerobic respiration and where they occur.
Glycolysis → cytoplasm
Link reaction → mitochondrial matrix
Krebs cycle → mitochondrial matrix
Oxidative phosphorylation → via electron transfer chain → membrane of cristae
Outline the stages of glycolysis.
Glucose is phosphorylated to glucose phosphate by 2x ATP
Glucose phosphate splits into 2x TP
2x TP is oxidised to 2x pyruvate
Net gain of 2x reduced of NAD and 2x ATP per glucose.
How does pyruvate from glycolysis enter the mitochondria.
Via active transport.
What happens during the link reaction?
Oxidation of pyruvate to acetate.
Per pyruvate molecule: net gain of 1x CO2 (decarboxylation) and 2H atoms (used to reduce 1x NAD).
Acetate combines with coenzyme A (CoA) to form acetylcoenzyme A.
Give a summary equation for the link reaction.
Pyruvate + NAD + CoA → acetyl CoA + reduced NAD + CO2
What happens in the Krebs cycle?
Series of redox reactions produces:
ATP by substrate-level phosphorylation.
Reduced coenzymes.
CO2 from decarboxylation.
What is the electron transfer chain (ETC)?
Series of carrier proteins embedded in membrane of the cristae of mitochondria.
Produces ATP through oxidative phosphorylation via chemiosmosis during aerobic respiration.
What happens in the electron transfer chain in respiration (ETC)?
Electrons released from reduced NAD and FAD undergo successive redox reactions.
The energy released is coupled to maintaining proton gradient or released as heat.
Oxygen acts as final electron acceptor.
How is a proton concentration gradient established during chemiosmosis in aerobic respiration?
Some energy released from the ETC is coupled to the active transport oh H+ ions from the mitochondrial matrix into the intermembrane space.
How does chemiosmosis produce ATP during aerobic respiration?
H+ ions move down their concentration gradient from the intermembrane space into the mitochondrial matrix via the channel protein ATP synthase.
ATP synthase catalyses ADP + Pi → ATP
State the role of oxygen in aerobic respiration.
Final electron acceptor in electron transfer chain.
(produces water as a byproduct).
What is the benefit of an electron transfer chain rather than a single reaction?
Energy is released gradually.
Less energy is released as heat.
Name 2 types of molecule that can be used as alternative respiratory substrates.
(amino acids from) proteins
(glycerol and fatty acids from) lipids
How can lipids act as an alternative respiratory substrate?
lipid → glycerol + fatty acid
Phosphorylation of glycerol → TP for glycolysis.
Fatty acid → acetate.
Acetate enters link reaction.
H atoms produced for oxidative phosphorylation.
How can amino acids act as an alternative respiratory substrate?
Deamination produces:
3C compounds → pyruvate for link reaction.
4C/5C compounds → intermediates in Krebs cycle.
Name the stages in respiration that produce ATP by substrate-level phosphorylation.
Glycolysis (anaerobic)
Krebs cycle (aerobic)
What happens during anaerobic respiration in animals?
Only glycolysis continues
reduced NAD + pyruvate → oxidised NAD (for further glycolysis) + lactate
What happens to the lactate produced in anaerobic respiration?
Transported to liver via bloodstream, where it is oxidised to pyruvate.
Can enter link reaction in liver cells or be converted to glycogen.
What happens during anaerobic respiration in some microorganisms?
Only glycolysis continues.
Pyruvate is decarboxylated to form ethanal.
Ethanal is reduced to ethanol using reduced NAD to produce oxidised NAD for further glycolysis.
What is the advantage of producing ethanol/ lactate during anaerobic respiration?
Converts reduced NAD back into NAD so glycolysis can continue.
What is the disadvantage of producing ethanol during anaerobic respiration?
Cells die when ethanol concentration is above 12%.
Ethanol dissolves cell membranes.
What is the disadvantage of producing lactate during anaerobic respiration?
Acidic, so decreases pH.
Results in muscle fatigue.
Similarities between aerobic and anaerobic respiration?
Both involve glycolysis.
Both require NAD.
Both produce ATP.
Differences between aerobic and anaerobic respiration.
Aerobic:
-produces ATP by substrate-level phosphorylation and oxidative phosphorylation
-produces much more ATP
-does not produce ethanol or lactate
Anaerobic
-substrate-level phosphorylation only
-produces fewer ATP
-produces ethanol or lactate