4-7 Flashcards
These are groups of microorganisms that live in aquatic habitats
Aeromonas
Infections caused are common among fish and amphibians
AEROMONAS
Gram-negative
Short, plump aerobic rods
aeromonas
Grow in trypticase soy agar at 22 to 25°C
aeromonas
Stools, bile and throat of avian, cattle, swine and dogs
feces and dysenteric stool of swine and dogs
aeromonas
transmission in aeromonas
ingestion of organism
Etiologic agent of Red Syndrome
A. hydrophila
Etiologic agent of Furunculosis
A. salmincola
Lethargy, emaciation, ulceration of the skin
red syndrome (a.hydrophila)
Systemic disease with ulceration
furunculosis (a. salmincola)
Crateriform abscesses that discharge contents to the skin (furuncle)
furunculosis (a. salmincola)
Hemorrhages on the fin, tail muscles, gills and intestinal organs
furunculosis (a. salmincola)
fin rot and ulceration of the skin
aeromonas
virulence factor of aeromonas
enterotoxins
adhesins
Rimler shotts medium
aeromonas
agent identification in aeromonaz
bacterial isolation and cultivation (trypticase soy agar)
Oxytetracycline
aeromonas
how many mg/kg intake of oxytetracycline?
60-75 in 21 days
give one control and prevention in aeromonas
- obtain fish and eggs from disease free sources
- stress reduction
Human pathogens that occasionally infect domestic animals
fransicella
3 species associated with francisella
- F. tularensis
- F. philomigaria
- F. novicida
• Fresh cultures possess capsules with high lipid and amino acid content
• Older cultures show pleomorphism
fransicella
which culture in francisella possess capsules with high lipid and amino acid content
fresh culture
which culture in fransicella show pleomorphism
older culture
Fastidious aerobes that prefer to grow in glucose-cysteine-blood agar
fransicella
Survives cold temperature in water, soila and animal lesions
fransicela
Reservoir of Infection
• Rabbits • Rodents
fransicella
Transmission
• Ingestion of infected prey, feed and water
• Bites of infected blood-sucking insects
fransicella
Systemic infection marked by ulcerative inflammatory and necrotic lesions
Tularemia (F. tularensis)
Nodules and cutaneous ulcer in tularemia infection
Tularemia
Virulence Factors
• Capsular lipids
fransicela
Preferred culture media: Glucose cystein blood agar
francisella
Agent identification
➢Bacterial cultivation
➢Guinea pig inoculation
➢PCR using primers for pathogenic
francisella
Treatment
• Streptomycin
• Tetracycline
• Aminoglycosides
fransicella
Control and prevention
• Limiting tick exposure and access to contaminated feed and water
francisella
growth factors (porphyrins or nicotinamide as X factor) and adenine dinucleotide (NAD, NADP) as V factor
➢Organisms exhibit satellite formation
hemophilus
Non-sporeforming, non-motile aerobic but some are facultative anaerobes
hemophilus
Capsules are composed of polysaccharides
hemophilus
• Organisms do not grow in plain or glycerol- containing agar
• Growth is enhanced by hemin and NAD and chocolate agar
hemophilus
Grows luxuriantly when a feeder bacterium is cross-streaked (satellism) with the organism
hemophilus
Reservoir of Infection
➢Respiratory tract (sick/carrier animals)
➢Nasopharynx (pigs)
➢Genital tracts (cattle and sheep)
hemophilus
secondary to viral infections {swine influenza} and other bacterial infections caused by Pasteurella and Mycoplasma spp.
Bronchopneumonia in animals
marked by sero-fibrinous to fibrino-purulent secretions in the lungs, body cavities and joints
Bronchopneumonia in animals
Swine influenza of swine
Glasser’s diseas (h. parasuis)
➢Common among young weaned pigs raised in stressful conditions
➢Bronchopneumonia secondary to bacterial and viral infections
Glasser’s Disease (H.parasuis)
Catarrhal inflammation of the upper respiratory tract
Coryza in chicken (H.paragallinarum)
marked by septicemia, meningoencephalitis and motor and behavioral abnormalities
Thrombotic meningo-encephalitis of cattle (H. somus)
Microorganisms are agents of local and septicemic infections of animals
actinobaccilus
Commensals of mucus membranes
➢ Opportunistic pathogens when host’s defense is compromised
actinobacillus
➢ Capsulated (A. pleuropneumonia) and non-capsulated
forms are present
➢ Some are piliated
actinobacillus
AfuA/actinoferric uptake)
➢ Form aggregates or clumps in small cheese-like grayish white sulfur granules
actinobacillus
• Reduce nitrates to nitrites
• Produce urease, ortho-nitro-phenyl-beta-D- galacto-pyranosidase and nitrite
actinobacillus
Transmission
• Endogenous infections
actinobacillus
• Chronic granulomatous infections in bovine tongue
• Colonies of A. lignieresii produce grayish white sulfur granules
Pyo-granuloma in ruminants
(Wooden Tongue - A. lignieresii)
• Abrasion and wounds penetrate the buccal mucosa result to formation of abscesses and tumors in the region of the lower jaw
and neck
• Pyogranulomatous lesions
in soft tissues (neck)
Wooden Tongue (A. ligrienesii)
• Pleuropneumonia in 2 to 6 month old pigs • Swollen joints
• Cough
Porcine pleuropneumonia (Resp Septicemia in Swine)
A. pleuropneumonia
Preferred Media: Blood agar under high amount of CO2
actinobacillus
Preferred Media: Blood agar under high amount of CO2
actinobacillus
crippling lameness in sheep goat and cattel
Foot rot (B. nodosus)
what antigens induce abscess fromation
capsular antigens
eugon agar
bacteroides
how many yeast in eugon agar
0.2%
how many horse blood in eugon agar
10%
in the presence of blood and serum
moraxella
grow best at 35 celcius
moraxella
grow in presence of blood and serum
moraxella
marked by epiphora, corneal clouding, ocular edema, vascularization and panophtalmitis
M. bovis (infectious Bovin Keratoconjunctivitis)
marked by sneezing, coughing, nasal and occulqr discharges with CNS involvement
New Duck disease (M. anatipestifer)
preferred culture media of moraxella
blood agar
sheep blood agar
listeria
These organisms are commonly found from sewage effluents abbatoirs, surface slimes of
fresh and salt water fishes and soil
erysipelothrix
Grow on solid medium containing glucose, serum, blood
erypsi