4/5/6 Mark Qs Genetic Information, Varyation & Relationships Between Organisms Flashcards
Compare and contrast the DNA in eukaryotic cells with the DNA in prokaryotic cells. (5)
Comparisons
1. Nucleotide structure is identical;
Accept labelled diagram or description of nucleotide as phosphate, deoxyribose and base
2. Nucleotides joined by phosphodiester bond;
OR
Deoxyribose joined to phosphate (in sugar, phosphate backbone);
3. DNA in mitochondria / chloroplasts same / similar (structure) to DNA in prokaryotes;
Accept shorter than nuclear DNA/is circular not linear/is not associated with protein/histones unlike nuclear DNA;
Contrasts
4. Eukaryotic DNA is longer;
5. Eukaryotic DNA contain introns, prokaryotic DNA does not;
6. Eukaryotic DNA is linear, prokaryotic DNA is circular;
7. Eukaryotic DNA is associated with / bound to protein / histones, prokaryotic DNA is not;
Compare and contrast the DNA in eukaryotic cells with the DNA in prokaryotic cells. (5)
Comparisons
1. Nucleotide structure is identical;
Accept labelled diagram or description of nucleotide as phosphate, deoxyribose and base
2. Nucleotides joined by phosphodiester bond;
OR
Deoxyribose joined to phosphate (in sugar, phosphate backbone);
3. DNA in mitochondria / chloroplasts same / similar (structure) to DNA in prokaryotes;
Accept shorter than nuclear DNA/is circular not linear/is not associated with protein/histones unlike nuclear DNA;
Contrasts
4. Eukaryotic DNA is longer;
5. Eukaryotic DNA contain introns, prokaryotic DNA does not;
6. Eukaryotic DNA is linear, prokaryotic DNA is circular;
7. Eukaryotic DNA is associated with / bound to protein / histones, prokaryotic DNA is not;
Describe how mRNA is formed by transcription in eukaryotes. (5)
- Hydrogen bonds (between DNA bases) break;
Ignore DNA helicase.
Reject hydrolysing hydrogen bonds. - (Only) one DNA strand acts as a template;
- (Free) RNA nucleotides align by complementary base pairing;
For ‘align by complementary base pairing’, accept ‘align to complementary bases’ or ‘align by base pairing’. - (In RNA) Uracil base pairs with adenine (on DNA)
OR
(In RNA) Uracil is used in place of thymine;
Do not credit use of letters alone for bases. - RNA polymerase joins (adjacent RNA) nucleotides;
Reject suggestions that RNA polymerase forms hydrogen bonds or joins complementary bases. - (By) phosphodiester bonds (between adjacent nucleotides);
- Pre-mRNA is spliced (to form mRNA)
OR
Introns are removed (to form mRNA);
Describe how a polypeptide is formed by translation of mRNA. (5)
. (mRNA attaches) to ribosomes
OR
(mRNA attaches) to rough endoplasmic reticulum;
2. (tRNA) anticodons (bind to) complementary (mRNA) codons;
3. tRNA brings a specific amino acid;
4. Amino acids join by peptide bonds;
5. (Amino acids join together) with the use of ATP;
6. tRNA released (after amino acid joined to polypeptide);
7. The ribosome moves along the mRNA to form the polypeptide;
Starting with mRNA in the cytoplasm, describe how translation leads to the
production of a polypeptide.
Do not include descriptions of transcription and splicing in your answer. (5)
mRNA associates with a ribosome / ribosome attaches to mRNA;
Idea of association is required
2. Ribosome moves to / finds the start codon / AUG;
3. tRNA brings / carries (appropriate / specific) amino acid;
Must be explicitly stated and not inferred.
4. Anticodon (on tRNA complementary) to codon (on mRNA);
5. Ribosome moves along to next codon;
OR
Ribosome ‘fits’ around two codons / can fit two tRNAs;
Must be explicitly stated and not inferred.
6. (Process repeated and) amino acids join by peptide bonds / condensation reaction (to form polypeptide);
OR
(Process repeated and) amino acids joined using (energy from) ATP (to form polypeptide);
Describe how mRNA is produced in a plant cell. (5)
. The DNA strands separate by breaking the H bonds;
OR
H bonds broken between (complementary) (DNA) bases;
2. (Only) one of the strands/template strand is used (to make mRNA/is transcribed);
3. (Complementary) base pairing so A ⟶ U, T ⟶ A, C ⟶ G, G ⟶ C;
4. (RNA) nucleotides joined by RNA polymerase;
5. pre-mRNA formed;
6. Splicing / introns removed to form mRNA;
1. Ignore ‘hydrolysis’ of bonds
1. Accept DNA “unzips” by breaking the H bonds
6. Accept ‘non-coding’ sections for introns
Crossing over greatly increases genetic diversity in this species of moss.
Describe the process of crossing over and explain how it increases genetic diversity. (4)
- Homologous pairs of chromosomes associate / form a bivalent;
- Chiasma(ta) form;
- (Equal) lengths of (non-sister) chromatids / alleles are exchanged;
- Producing new combinations of alleles;
- Accept descriptions of homologous pairs
- Accept descriptions of chiasma(ta) e.g. chromatids / chromosomes entangle / twist
- Neutral Crossing / cross over
- Reject genes are exchanged
- Accept lengths of DNA are exchanged
- Do not accept references to new combinations of genes unless qualified by alleles