39: Molecular DNA Flashcards
branch of biology that addresses how differences in the structures of expression of DNA molecules manifests as a variation among organisms
Molecular Genetics
study of molecular structure of DNA, its cellular activities and its influence in determining the overall make-up of an organism
Molecular Genetics
relies heavily on genetic engineering, which can be used to modify organisms by adding foreign DNA, thereby forming transgenic organism
Molecular Genetics
involves isolation and manipulation of DNA to make chimeric molecules
Recombinant DNA
a molecule (DNA, RNA and protein) containing sequences derived from 2 different species
Chimeric molecule
2 or more components are linked to form a novel biological agent
Chimeric molecule
the development of technology to sequence the DNA of whole genomes on a routine basis
Genomic technology
involves using enzyme and various laboratory techniques to manipulate and isolate DNA segments of interests
recombinant DNA technology
used to engineer and prepare chimeric DNA molecules
- Restriction enzymes
- Endonucleases
- Recombinases
- Thermostable DNA polymerase
- DNA synthesizers
- DNA ligases
key tools in recombinant DNA
Restriction enzymes
their presence in a given bacterium restricted or prevented the growth of certain bacterial virus called bacteriophages
Restriction enzymes
cut DNA of any source into unique short places in a sequence-specific manner
Restriction enzymes
present only in cells that also have a companion enzyme that site-specifically methylates the DNA of bacterial host, thereby rendering it non-cleavable by particular restriction enzyme
restriction endonucleases
named after the bacterium from which they are isolated
restriction enzymes
