34 Antibody-based Drugs

Question 1

2 fates of naive Th cells

Answer 1

Th1 = activate CD8 cytotoxic T-cells
Th2 = activate B-cells

Question 2

2 methods to make mAbs

Answer 2

Hybridoma

Phage-display

Question 3

Explain phage display method

Answer 3

Clone mAb sequences into phage
Form bacteriophage (exp ab on plasmid)
Expose to target antigen
ELUTE + amplify the abs that bind

Question 4

Acronym for the 5 antibodies studied

Name these and their targets

Answer 4

The Pig Ran Behind the Cat

Traztuzumab - HER2
Pertuzumab - HER2/3
Rituximab - CD20
Bevacizumab - VEGFA
Cetuximab - EGFR

Question 5

Which of the 5 antibodies (TPRBC) are humanised?

Which are chimeric?

Answer 5

RC = chimeric

Question 6

MoA of traztuzumab killing cells?

Answer 6

ADCC = Antibody-Dependent Cell-mediated Cytotoxicity

Question 7

Acronym for the immune checkpoint inhibitors

Name these and their targets

Answer 7

the girl from IPNA

Ipilimumab - CTLA-4
Pembrolizumab - PD-1
Nivolumab - PD-1
Atezolizumab - PD-L1

Question 8

What are ADCs

Name 2

Answer 8

Antibody-Drug Conjugates

traztuzumab-emtansine
Brentuximab-vedotin

Question 9

Name the 3 components of both ADCs

Answer 9

mAb: traztuzumab
Linker: thioether linker (MCC)
Drug: emtansine (MT inhibitor)

mAb: brentuximab
Linker: valine-citruline
Drug: vedotin (MT inhibitor)

Question 10

Target of brentuximab?

Answer 10

CD30