3.2

Question 1

5 Factors affecting enzyme action?

Answer 1

Factors that can affect enzyme action include:
• Temperature
• pH
• Concentration
• Surface area
• Pressure

Question 2

What will happen if the temperature rises past the optimum temperature?

Answer 2

As the temperature rises past the optimum the hydrogen bonds in the enzyme molecule will break, the active site loses its specific 3D shape so that the substrate can no longer fit as easily. The enzyme becomes permanently denatured.

Question 3

What happens at low substrate concentration?

Answer 3

1. At low substrate concentration, the enzymes only have a limited number of substrates to collide with – the active sites of the enzymes are not working at maximum capacity.

Question 4

What happens if more substrate is added to a low substance concentration enzyme?

Answer 4

2. As more substrate is added, the active site becomes more filled, until they are all working at their maximum – this is called the Vmax.

Question 5

What happens if more sub starter is added surpassing the Vmax

Answer 5

3. After this, adding more substrate will have no effect on the rate of reaction and so the reaction will level off.

Question 6

State the method for the investigation of the effect of temperature on amylase enzyme

Answer 6

1) iodine added to spotting tile
2)place a test tube containing amylase and a second containing starch into a water bath to reach the required temperature
3) add the amylase to the starch and start the timer
4) after ten seconds add a drop of solution to iodine in spitting tile
5) repeat every ten seconds until iodine doesn’t change color
6) repeat steps 1-5 at different temperatures