3.1.4.1 Proteins - structure, function and biuret test Flashcards
What decides the amino acid sequence that determines the primary structure of a protein?
A DNA sequence
Describe the ionic bonds that hold the tertiary structure of a protein in place.
Weaker than disulfide bonds
Easily broken by changes in pH
Form between free carboxyl and amino groups in the polypeptide chain
What does Biuret reagent contain?
Concentrated Copper (II) sulfate solution
Describe the disulfide bonds that hold the tertiary structure of a protein in place.
Strong
Not easily broken
Occur between two cysteine amino acids
Name the bonds that hold the tertiary structure of a protein in place.
Disulfide Bonds
Hydrogen Bonds
Ionic Bonds
What do the below instructions describe:
- Place a 2cm3 of food in a test tube.
- Add 2 cm3 of sodium hydroxide solution at room temperature.
- Add a few drops of very dilute copper (II) sulfate solution (Biuret reagent)
- Observe and record colour change.
The Biuret test for proteins.
Identify the molecule
Amino acid
Name 3 fibrous type proteins
Collagen
Keratin
Silk
What is the monomer unit of a protein?
Amino acid
What do we call 2 amino acids chemically joined together?
Dipeptide
What is meant by a fibrous protein?
A protein made from many parallel polypeptide chains. These chains main form an α helix shape.
Fibrous proteins are usually insoluble in water.
Which colour does Biuret solution turn in the presence of protein?
Biuret changes from blue to purple.
Give 3 examples of enzymes
Amylase
Protease
Lipase
Which colour does Biuret reagent turn in the absence of protein?
It remains a blue colour
Identify the circled group
R group
Idenitfy the circled group
Amine group
What is meant by the tertiary structure of a protein?
When the α helix or β pleated sheets of the secondary protein structure are folded even more to give a complex, specific 3D structure.
What colour is Biuret reagent?
Blue