2.9 Photosynthesis Flashcards
Photosynthesis
the process by which cells synthesise organic compounds from inorganic molecules in the presence of sunlight
Photosynthesis equation
6CO2 + 6H2O =(light + chlorophyll)> C6H12O6 + 6O2
Photosynthesis word equation
carbon dioxide + water => glucose + oxygen
What is light energy used for?
create chemical energy (ATP)
The electromagnetic spectrum light
Roy G. Biv red orange yellow green blue indigo violet
Chlorophyll
green pigment found in photosynthetic organisms that is responsible for light absorption
What light does chlorophyll absorb the most?
blue then red
What light does chlorophyll reflect the most?
green
Absorption spectrum
indicates the wavelengths of light absorbed by each pigment
Action spectrum
indicates the overall rate of photosynthesis at each wavelength of light
What are the two steps of photosynthesis?
light dependent reactions convert light energy from the Sun into chemical energy (ATP)
light independent reactions use the chemical energy to synthesise organic compounds
Chromatography
an experimental technique by which mixtures can be separated
Paper chromatography
uses paper (cellulose) as the stationary bed
Thin layer chromatography
uses a thin layer of adsorbent which runs faster and has better separation
What conditions is photosynthesis dependent on?
temperature
light intensity
carbon dioxide concentration
Effect of temperature on photosynthesis
photosynthesis is controlled by enzymes, which are sensitive to temperature fluctuations
temp increases - reaction increases as more kinetic energy results in more collisions
above a certain temperature the rate of photosynthesis will decrease
Effect of light intensity on photosynthesis
as light intensity increases reaction rate will increase, as more chlorophyll are being photo-activated
at a certain light intensity photosynthetic rate will start to stop, as all available chlorophyll are saturated with light
(different wavelengths of light will have different effects)
Effect of carbon dioxide concentration on photosynthesis
as carbon dioxide concentration increases reaction rate will increase, as more organic molecules are being produced
at a certain concentration of CO2 photosynthetic rate will plateau, as the enzymes responsible for carbon fixation are saturated
How can we measure rate of photosynthesis?
uptake of carbon dioxide
production of oxygen
a change in biomass
How to measure carbon dioxide uptake?
place leaf tissue in an enclosed space with water (free of carbon dioxide by boiling and cooling)
carbon dioxide interacts with the water molecules (produces bicarbonate and hydrogen ions) and changes the pH
increased uptake of carbon dioxide will lower the concentration in solution and increase alkalinity
How to measure oxygen production?
submerging a plant in an enclosed water-filled space attached to a sealed gas syringe
any oxygen gas produced will bubble out of solution and can be measured by a change in meniscus level on the syringe
How to measure biomass?
measure the change in the plant’s biomass (after completely dehydrated)