27: Pasteurella spp. Mannheimia haemolytica, Bibersteinia trehalosi Flashcards
Oxidase
Positive
Environment
Facultative anaerobes
Grows best on
Enriched media (blood/serum supplemented)
Can tolerate bile salts in MacConkey agar
Mannheimia haemolytica
Bibersteinia trehalosi
Pasteurella aerogenes
Family
Pasteurellaceae
Staining
Pasteurellae- bipolar staining in Giemsa method
Major pathogens
P. multocida
M. haemolytica
B. trehalosi
Habitat
Ommendals on upper respiratory tract (mucosae)
-Round, greyish, shiny, non-haemolytic
-some are mucoid (thick hyaluronic acid)
P. multocida
sweetish odour
P. multocida
Haemolytic and odorless
M. haemolytica
M. granulomatous
B. trehalosi
Round, greyish, non-haemolytic (except)
Pasteurella spp. ( P. testudinis)
MacConkey agar- Pasteurella multocida
Do not grow
MacConkey agar- M. haemolytica, B. trehalosi
pin-point, red colonies
Infections are
Endogenous
Adhesins
-Fimbriae
-Surface fibrils
-Filamentous haemagglutinins
-Capsule…
Type A and B capsule
Hyaluronic acid
Type D capsule
Heparin
Type F
Chondroitin
P. multocida type D major virulence
PMT toxin- atrophic rhinitis in pigs
M. haemolytica major adhesin
-OmpA
-Lpp1