27: Pasteurella spp. Mannheimia haemolytica, Bibersteinia trehalosi

Question 1

Oxidase

Answer 1

Positive

Question 2

Environment

Answer 2

Facultative anaerobes

Question 3

Grows best on

Answer 3

Enriched media (blood/serum supplemented)

Question 4

Can tolerate bile salts in MacConkey agar

Answer 4

Mannheimia haemolytica
Bibersteinia trehalosi
Pasteurella aerogenes

Question 5

Family

Answer 5

Pasteurellaceae

Question 6

Staining

Answer 6

Pasteurellae- bipolar staining in Giemsa method

Question 7

Major pathogens

Answer 7

P. multocida
M. haemolytica
B. trehalosi

Question 8

Habitat

Answer 8

Ommendals on upper respiratory tract (mucosae)

Question 9

-Round, greyish, shiny, non-haemolytic
-some are mucoid (thick hyaluronic acid)

Answer 9

P. multocida

Question 10

sweetish odour

Answer 10

P. multocida

Question 11

Haemolytic and odorless

Answer 11

M. haemolytica
M. granulomatous
B. trehalosi

Question 12

Round, greyish, non-haemolytic (except)

Answer 12

Pasteurella spp. ( P. testudinis)

Question 13

MacConkey agar- Pasteurella multocida

Answer 13

Do not grow

Question 14

MacConkey agar- M. haemolytica, B. trehalosi

Answer 14

pin-point, red colonies

Question 15

Infections are

Answer 15

Endogenous

Question 16

Adhesins

Answer 16

-Fimbriae
-Surface fibrils
-Filamentous haemagglutinins
-Capsule…

Question 17

Type A and B capsule

Answer 17

Hyaluronic acid

Question 18

Type D capsule

Answer 18

Heparin

Question 19

Type F

Answer 19

Chondroitin

Question 20

P. multocida type D major virulence

Answer 20

PMT toxin- atrophic rhinitis in pigs

Question 21

M. haemolytica major adhesin

Answer 21

-OmpA
-Lpp1

Question 22

M. haemolytica and B. trehalosi 4 major virulence

Answer 22

-Adhesins
-Capsule
-Endotoxin
-Leukotoxin
(ACE-L)

Question 23

Shipping fever

Answer 23

-P. multocida type A
-M. haemolytica

Question 24

Atrophic rhinits

Answer 24

Pigs
P. multocida type A and D

Question 25

Snuffles

Answer 25

Rabbit P. multocida type A

Question 26

Fowl cholera

Answer 26

Poultry Type A and F (turkeys)

Question 27

Associated with enzootic pneumonia complex in calves

Answer 27

P. multocida type A

Question 28

Haemorrhagic septicaemia (Asia)

Answer 28

P. multocida type B

Question 29

Rare cases of peritonitis

Answer 29

Calves Type F

Question 30

Septicaemia under 3 months age

Answer 30

Sheep M. haemolytica

Question 31

Septicaemia 5-13 months of age

Answer 31

Sheep Bibersteinia trehalosi

Question 32

Gangrenous mastitis

Answer 32

M. haemolytica

Question 33

Haemorrhagic septicaemia (Africa)

Answer 33

Cattle and buffaloes Type E

Question 34

Fibrogranulomatous panniculitis

Answer 34

Cattle M. granulomatis

Question 35

Lymphangitis (rare)

Answer 35

Cattle P. lymphangitidis

Question 36

Found in respiratory tract

Answer 36

Dogs and cats P. stomatis

Question 37

Found in intestine

Answer 37

Ducks P. anatis

Question 38

Commensals in oral cavity and nasopharynx

Answer 38

Dogs and cats P. dagmatis

Question 39

Positive on haemolysis on sheep blood agar

Answer 39

M. haemolytica B. trehalosi

Question 40

Positive for MacConkey

Answer 40

M. haemolytica B. trehalosi

Question 41

Positive for distinctive odor colonies

Answer 41

P. multocida

Question 42

Positive for indole

Answer 42

P. multocida P. pneumotropica

Question 43

Positive for catalase

Answer 43

Except B. trehalosi

Question 44

Positive for urease

Answer 44

Only P. pnemotropica

Question 45

Positive for ormithine decarboxylase activity

Answer 45

P. multocida P. pneumotropica

Question 46

Lactose positive

Answer 46

M. haemolytica

Question 47

Positive for sucrose

Answer 47

All

Question 48

Positive for D-trehalose

Answer 48

B. trehalosi P. pnemotropica P. multocida (variable)

Question 49

Positive for L-arabinose

Answer 49

None P. multocida (variable)

Question 50

Positive for maltose

Answer 50

M. haemolytica B. trehalosi

Question 51

Positive for D-xylose

Answer 51

M. haemolytica (+) P. multocida (v) B. trehalosi (-) P. pneumotropica (v)

Question 52

Species included (major pathogens)

Answer 52

P. multocida B. trehalosi M. haemolytica

Question 53

Environment

Answer 53

Facultative anaerobes

Question 54

Motility

Answer 54

Non motile