2.5 Enzymes Flashcards
1
Q
Enzyme
A
- A globular protein that increases the rate of a biochemical reaction by lowering the activation energy threshold (i.e. a biological catalyst)
2
Q
Active Site
A
- Region on the surface of an enzyme to which the substrates bind and which catalyzes the reaction.
3
Q
Substrates
A
- Substances that enzymes convert into products in these reactions (the reactants).
4
Q
Products
A
- The end result.
5
Q
Lock and Key model
A
- The first theory of the action of an enzyme binding to a substrate.
6
Q
Induced Fit Model
A
-The current theory of the action of an enzyme binding to a substrate.
7
Q
Enzyme-substrate specificity
A
- A feature of an enzyme activity with regard to the kind of substrate reacting with an enzyme to yield a product.
8
Q
State what is meant by the term collision in enzyme catalysis.
A
- The coming together of a substrate molecule and an active site is known as a collision.
9
Q
Explain why not all collisions between enzymes and substrates result in catalysis.
A
- As not all collisions result in binding.
10
Q
Explain why the presence of water is critical for most enzyme reactions.
A
- Most enzyme reactions occur when the substrates are dissolved in water.
- All molecules dissolved in water are in random motion, with each molecule moving separately.
11
Q
Enzymes can be immobilized (e.g. embedded in cell membranes). Enzymes can also be free to move, but they tend to move more slowly than substrates, explain why.
A
- If not immobilized the enzyme can move too, however enzymes tend be larger than the substrate(s) and therefore move more slowly.
12
Q
- Effects of Temperature on Rate of Reaction
A
- Low temperatures result in insufficient thermal energy for the activation of a given enzyme-catalysed reaction to be achieved.
- Increasing the temperature will increase the speed and motion of both enzyme and substrate, resulting in higher enzyme activity.
- This is because a higher kinetic energy will result in more frequent collisions between enzyme and substrate.
- At an optimal temperature (may differ for different enzymes), the rate of enzyme activity will be at its peak.
- Higher temperatures will cause enzyme stability to decrease, as the thermal energy disrupts the hydrogen bonds holding the enzyme together.
- This causes the enzyme (particularly the active site) to lose its shape, resulting in a loss of enzyme activity (denaturation).
13
Q
Effects of pH on Rate of Reaction
A
- Changing the pH will alter the charge of the enzyme, which in turn will protein solubility and may change the shape of the molecule.
- Changing the shape or charge of the active site will diminish its ability to bind to the substrate, halting enzyme function.
- Enzymes have an optimum pH and moving outside of this range will always result in a diminished rate of reaction.
- Different enzymes may have a different optimum pH ranges.
14
Q
Effects of Substrate Concentration on Rate of Reaction
A
- Increasing Substrate Concentration increases Rate of Reaction.
- At optimum concentration of substrate molecules, all active sites are full and working at maximum efficiency.
- Any increase in concentration beyond the optimum, will have no added effect as there are no extra active sites to be used.
15
Q
List eight common uses of enzymes in industry.
A
- Detergents.
- Food Industry.
- Paper production.
- Textiles.
- Brewing.
- Medicine & Biotechnology.
- Biofuels.