2.4.6 the effect of substrate conc on the rate of enzyme-catalysed reactions

Question 1

if no substrate… because…

Answer 1

• no enzyme-catalysed reaction occurs
• no substrate molecules to fit into complementary enzymes active site = no ESC formed

Question 2

as substrates added (& conc. increases), rate of reaction increases because

Answer 2

• more ESC can form = more product molecules formed

Question 3

what is the limiting factor in this reaction

Answer 3

substrate conc. because as it increases so does the rate

Question 4

as the conc. of substrate is further increased, what will the reaction reach

Answer 4

maximum rate

Question 5

what does it mean if a reaction is at maximum rate

Answer 5

• adding more substrate molecules (increase conc.) will not increase the rate as all enzyme’s active sites are occupied
• they cannot successfully collide & fit into an enzyme’s active site