2.4 - Enzymes Flashcards
What is an Enzyme?
An enzyme is a globular protein which catalyses chemical reactions and speeds up metabolic reactions.
What makes an Enzyme so special?
Chemical catalysts, which provide a similar role to enzymes, usually require very high temperatures and extreme values of pH in order for them to function efficiently. An enzyme can speed up reactions in life sustaining conditions , do not produce unwanted products and do not usually make any errors.
What is an active site?
This is a cleft in the surface of the enzyme. The tertiary structure of the active site is complementary to the shape of the substrate molecule. This increases the specificity of the enzyme.
What is an anabolic enzyme?
This is an enzyme which joins substrates together.
What is a catabolic enzyme?
This is an enzyme which breaks down substrates.
What is an intracellular enzyme?
These are enzymes which catalyse within the cell they are produced. i.e. ribosomes, DNA helicase and DNA polymerase.
What is a metabolic pathway?
In a cell many metabolic reactions occur simultaneously. Some of these follow a pathway where the product of one enzyme-substrate complex is the substrate for another. An example of a metabolic pathway is respiration.
What is an example of an intracellular enzyme?
CATALASE is an enzyme which is found in nearly in all living organisms which are exposed to oxygen. It protects a cell from damage by breaking down HYDROGEN PEROXIDE. 2H2O2 → O2 + 2H2O. Catalase is made up of four polypeptide chains and an iron haem group. In a eukaryotic cell it is found in small vesicles which are called PEROXISOMES.
What is an extracellular enzyme?
These are enzymes which are secreted from the cell that they are produced in. This is particularly used in the digestive system where enzymes are used to break down food.
What is an example of an extracellular enzyme?
AMYLASE is produced in the SALIVARY GLANDS and acts in the MOUTH in order to break down the polysaccharide STARCH into the disaccharide MALTOSE.It is also made in the pancreas where it catalyses the same reaction in the lumen of the small intestine.
TRYPSIN is made in the pancreas and acts in the lumen of the SMALL INTESTINE to break down PROTEINS into PEPTIDES.
What is a cofactor?
Some enzymes, especially the on which catalyse redox reactions, only work if another SMALL NON- PROTEIN MOLECULE is attached to the enzyme. For example a CHLORINE ION (Cl-) is the cofactor for AMYLASE.
What is a prosthetic group?
This is a cofactor which is PERMANENTLY bound to the enzyme by a covalent bond./ For example, CARBONIC ANHYDRASE contains a ZINC ION (ZN+) prosthetic group. It catalyses the interconversion of CO2 and H20 to H2CO3 (Carbonic acid) which breaks down to protons (H+) and Hydrogen Carbonate ions (HCO3-). This is important as it enables CO2 to be carried in the blood.
What is a coenzyme?
These are SMALL ORGANIC NON - PROTEIN molecules, which are a type of cofactor, that bind temporarily to the active site either just before or at the same time as when the substrate binds to the enzyme. During this process the coenzyme is chemically changed and are then recycled.
Can you name a source of coenzymes?
Many coenzymes can be found in vitamins. For example Nicotinamide provides the coenzymes NAD and NADP which may cause diarrhoea , dementia etc. if there is a deficiency.
What is the ‘lock and key’ hypothesis?
The random movement of the enzyme and substrate brings the substrate into the active site. The substrate molecule fits the tertiary structure of the active site as it completely specific to that substrate - like a lock and key.
An enzyme substrate complex is temporarily formed by hydrogen bonds when the R groups in the amino acids of the active site interacts with the substrate. The substrate molecule is either broken down or built into a larger product molecule. The product molecule leaves the active site.