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Microscopes > 2.2.1 Microscopes > Flashcards

2.2.1 Microscopes Flashcards

1
Q

What are some advantages of light microscopes

A
  • Cheap
  • Easy to use
  • Portable
  • Can view living species
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2
Q

What cannot be observed with a light microscope?

A

Non membrane bound cells eg.ribosomes

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3
Q

What are some disadvantages with Laser Scanning Microscopes

A
  • Expensive
  • Not portable
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4
Q

What are Laser Scanning Microscopes used to observe?

A

Living specimens as well as cells

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5
Q

What is the magnification in light microscopes?

A

x1500 or in some x2000

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6
Q

What is the resolution in light microscopes?

A

200nm

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7
Q

Does Laser Scanning Microscopes have high or low resolution?

A

High

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7
Q

Does Laser Scanning Microscopes have high or low resolution?

A

High

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8
Q

What is the magnification is Transmission Electron Microscope?

A

x2,000,000

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9
Q

What is the resolution in Transmission Electron Microscope?

A

0.2nm

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10
Q

What is an advantage of Transmission Electron Microscope?

A

A very high magnification

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11
Q

What are some disadvantages with Transmission Electron Microscopes?

A
  • Black and white image produced
  • Specimen has to be dead
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12
Q

How does Electron Transmission Microscope work?

A
  • Specimen chemically fixed by being dehydrated and stained (stained with metal salts)
  • Beam of electrons pass through specimen
    -2D black and white image produced
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13
Q

What are some differences between the images produced with Transmission Electron Microscope compared to Scanning Electron Microscope?

A

TEM - 2D black and white image produced
SEM - 3D coloured image can be produced

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14
Q

What is the magnification for Scanning Electron Microscopes?

A

x15 to x200,000

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15
Q

What is the resolution for SEM?

A

3nm to 20nm

16
Q

What are some advantages of SEM?

A
  • Can create a 3D image
  • Colour can be added to image through computer software programmes
17
Q

What are some disadvantages of SEM?

A
  • Very large so is not portable
  • Very expensive
  • Needs a lot of skill and training to use
18
Q

Definition for electron microscope

A

photograph of an image seen using an electron microscope

19
Q

Definition of magnification

A

The number of times larger an image appears, compared with the size of the object

20
Q

Definition of organelles

A

Small structures within cells, each of which has a special function

21
Q

Definition of photomicrograph?

A

Photograph of an image seen using an optical microscope

22
Q

Definition of resolution

A

The clarity of an image, the higher the resolution the clearer the image

Microscopes (2 decks)

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