2.1.5 Biological membranes Flashcards
Role of membranes at surface of cell (plasma membranes)
barrier between the cell and its environment
- control which substances enter and leave the cell
partially permeable, let some molecules through but not others
- can more across membrane by diffusion, osmosis or active transport
cell recognition eg. the cells of the immune system
cell communication (cell signalling)
Role of membranes within cells
compartmentalise
- membranes around organelles divide them into different compartments
- this makes functions more efficient
eg. lysosomes contain digestive enzymes within their membrane
can form vesicles
- to transport substances between different areas of the cell
control which substances enter and leave the organelle
- partially permeable
membranes within organelles
- act as barriers between membrane and contents of the rest of the organelle
eg. thylakoid membrane in chloroplasts
site of chemical reactions
eg. inner membrane of a mitochondrion contains enzymes needed for respiration
Describe the fluid mosaic model of membrane structure
- phospholipid molecules from a continuous double layer (bilayer)
- this bilayer is fluid as phospholipids constantly moving
- cholesterol molecules are present within the bilayer
- protein molecules are scattered through the bilayer
- glycoproteins have a polysaccharide chain attached - glycolipid molecules - lipids with a polysaccharide chain attached
Roles of components in membrane - phospholipids
Have a head and a tail
- head is hydrophilic it attracts water
- tail is hydrophobic it repels water
the molecules automatically arrange themselves in a bilayer (heads face outwards)
the centre of the bilayer is hydrophobic
- doesn’t allow water soluble substances (ions) though it, its acts as a barrier
- fat soluble substances can dissolve in the bilayer and pass directly through the membrane
Roles of components in membrane - cholesterol
a type of lipid
in all cell membranes (except bacteria)
fit between phospholipids
- they bind to the hydrophobic tails of the phospholipids
- this causes them to pack more closely together
this makes the membrane less fluid and more rigid
Roles of components in membrane - intrinsic proteins
control what enters and leaves the cell
channel proteins
- allow small or charged particles through (diffusion)
carrier proteins and transport molecules
- active transport
- facilitated diffusion
Roles of components in membrane - extrinsic proteins
glycolipids and glycoproteins
stabalise membrane by forming hydrogen bonds with surrounding water molecules
the sites where drugs hormones and antibodies bind
act as receptors for cell signaling
antigens - cell surface molecules involves in the immune system
how do cells communicate with each other?
by cell signalling
one cell releases a messenger molecule (eg. a hormone)
this molecule travels (eg. in the blood) to another cell
the messenger molecule is detected by the cell because it binds to a receptor on its cell membrane
Why do cells need to communicate with each other?
to control processes inside the body and to respond to changes in the environment
How are plasma membranes involved in cell signalling?
proteins in the membrane act as receptors for messenger molecules
receptor proteins have specific shapes, only a messenger molecule with a complementary shape can bind to them (target cells)
different cells have different types of receptors, they respond to different messenger molecules
How do drugs work?
by binding to receptors in cell membranes
they either trigger a response or block the receptor and prevent it from working
Factors affecting membrane permeability
temperature
solvents
How does temperature affect membrane affect solubility? - below 0degrees
phospholipids dont not have much energy so cannot move very much
packed closely together and the membrane is rigid
channel proteins and carrier proteins deform - INCREASING PERMEABILITY
ice crystals may form
- this pierces the membrane making it highly permeable when it thaws
How does temperature affect membrane affect solubility? - between 0 and 45 degrees
the phospholipids can move around and arent as tightly packed
- the membrane is partially permeable
as temp. increases the phospholipids move more (have more kinetic energy)
- this INCREASES PERMEABILITY of the membrane
How does temperature affect membrane affect solubility? - above 45degrees
the phospholipid bilayer starts to melt (break down)
- membrane MORE PERMEABLE
water inside the cell expands putting pressure on the membrane
channel and carrier proteins deform (denature) so cannot control when enters or leaves the cell - INCREASING PERMEABILITY
How does changing the solvent affect membrane permeability?
surrounding cells in a solvent INCREASES PERMEABILITY of the membrane
- solvents dissolve the lipids in the membrane so it loses its structure
- some solvents increase permeability more than others
- INCREASING SOLVENT CONC. INCREASES PERMEABILITY
Experiment to measure permeability of the membrane
using beetroot
1. cut equal-sized pieces of beetroot and rinse them (removed pigment released during cutting)
- place in five different test tubes
- temp –> same vol. of water and put in water baths for the same length of time
- solvent –> same vol. of different concs. same length of time (poss. same temp. water bath) - remove pieces of beetroot from the tubes leaving just the coloured liquid
- use a colorimeter to measure absorbance
- the higher the permeability of the membrane the more pigment is released so the higher the absorbance of the liquid
Transport across membranes - passive movement
doesn’t require ATP (energy from metabolic processes)
- diffusion
- facilitated diffusion