2.1.4 Enzymes

Question 1

Role of enzymes

Answer 1

They catalyse reactions and therefore affect metabolism at a cellular and whole organism level

Question 2

Role of enzymes in catalysing both
intracellular and extracellular reactions

Answer 2

Include catalase as an example of an enzyme that catalyses intracellular reactions and amylase as example of an enzyme that catalyses extracellular
reactions.

Question 3

Mechanism of enzyme action
- tertiary structure, specificity,

Answer 3

enzyme-substrate complex, enzymeproduct complex, product formation

Question 4

Lock and Key hypothesis

Question 5

Induced fit hypothesis

Question 6

lowering of
activation energy

Question 7

The effects of pH
on enzyme activity

Question 8

The effects of temperature on enzyme activity

Question 9

Temperature coefficient

Question 10

The effects of enzyme
concentration on enzyme activity

Question 11

The effects of substrate concentration on enzyme activity

Question 12

Cofactors

Answer 12

Chloride ion as a cofactor for amylase

Question 13

Co enzymes

Answer 13

Vitamins as a source of coenzymes

Question 14

Effects of inhibitors on the rate of enzymecontrolled reactions

Question 15

Competitive and non-competitive inhibitors

Question 16

reversible and non-reversible inhibitors

Question 17

**

The role
of end-product inhibition.