2.1.4 enzymes Flashcards
function of enzymes
they are biological catalysts
they function in living system and speed up the rate of chemical reactions without being used up or undergoing permanent change
structure of enzymes
globular proteins with complex tertiary structures
- some formed from a single polypeptide
- others made up of two or more polypeptides - quaternary structure
what do enzymes control in a reaction?
- metabolic pathways are controlled by enzymes
- every metabolic reaction in a living organism is catalysed by an enzyme
- enzymes are essential for life
how are enzymes produced?
enzymes are proteins that are produced via protein synthesis inside cells
how can enzymes work?
intracellularly and extracellularly
what are intracellular enzymes?
produced and function inside the cell
what are extracellular enzymes
secreted by cells and catalyse reactions outside cells
what is the temperature coefficient?
the ratio between the rates of that reaction at two different temperatures
temperature coefficient for enzyme-catalysed reactions
the rate of the reaction doubles for every 10oC increase in temp
Q10 = 2
temperature coefficient equation
temperature coefficient = (rate of reaction at (x+10) oC / (rate of reaction at xoC)
what happens when enzymes are denatured at extreme pH?
- hydrogen and ionic bonds hold the tertiary structure of the protein together
- below and above the optimum pH of an enzyme, solutions with an excess of H+ ions and OH- ions causes these bonds to break
- the breaking of bonds alters the shape of the active site so that the enzyme-substrate complex forms less easily
- then it can no longer form and complete denaturation has occurred
explain how pepsin functions
pepsin is found in the stomach, an acidic environment at pH 2 - due to the presence of HCl in the stomach
pepsin’s optimum pH is pH 2
how can you use buffer solutions to measure the rate of reaction at different pH values?
- buffer solutions have a specific pH
- they maintain this pH
- a measured volume of the buffer solution is added to the reaction mixture
- this same volume should be added for each pH value that is being investigated
investigating the effect of pH on enzyme reaction rates
- use the enzyme amylase to breakdown starch at different pH values, using iodine as an indicator for the reaction occurring
- a continuous sampling technique can monitor the progress of the reaction
method for the investigation into the effect of pH on enzyme reaction rates
- wear goggles and gloves - enzymes may cause allergic reactions when in contact with skin
- place drops of iodine on the tile
- label a test tube with the pH to be tested
- use the syringe to place 2cm3 of amylase in the test tube
- add 1cm3 of buffer solution to test tube
- use another test tube to add 2cm3 of starch solution to the solution and start the stopwatch whilst mixing
- use pipette to place a drop of solution onto iodine (should turn blue-black in the presence of starch)
- place another drop of solution onto iodine and repeat every 10 seconds (until solution is orange-brown - no presence of starch)
- repeat experiment at different pH values
control variables in the enzyme reaction rates practical
equal volumes and concentrations of enzyme used
equal volumes and concentrations of substrate used
what does it mean when the solution no longer turns blue-black?
no starch present
amylase has broken down starch so there is nothing left to react with iodine