2.1.1 Cell Structure Flashcards
A)What are the microscopes you need to know
- light/optical microscopes
- Transmission electron microscopes
- Scanning electron microscopes
A)what does a light ( optical ) microscope do
- they helps to understand cell structure
- optical microscopes uses light (wavelength 400-700 nm) which is focused by glass.
- the photo of the image produced is called a photomicrograph
A) what are the advantages of using a light / optical microscopes
- linear magnification (x50 means 50 times larger)
- cheap
- easy to use
- portable
- stains are not necessary
A) what are the disadvantages of a light/optical microscope
- limited resolution so some organelles cannot be seen ( eg ribosomes)
A) how does a transmission electron microscope work
- a beam of electron ( wavelength 0.004nm) from a cathode passes through the specimen and are focused by magnets onto a screen/photographic plate
- produces a 2B black and white photomicrograph
A) what are the advantages to transmission electron microscopes
- very high magnification ( x2 000 000 max)
- 0.1 nm resolution
- Ultrastructure seen
A) what are the disadvantages of transmission electron microscopes
- large
- expensive
- needs skills and training to use
- specimen needs to be in a vacuum
-specimens needs to be dehydrated and stained with metal salt (may be dangerous)
A) How does a scanning electron microscope work
- use of a beam of electrons from a cathode ( 0.004nm wavelength) that bounces of a specimen and then focussed by magnets.
- this makes a 3D black and white electron micrograph ( colour can be added by computer)
A) what are the advantages of using a scanning electron microscope
- high magnification achieved (x15 - x200,000)
- 0.1 nm resolution
- whole specimen can be used
A) what are the disadvantages of scanning electron microscopes
- large
- expensive
- need skill and training to use
- cannot be used to study living organisms
- specimen needs to be viewed while in a vacuum
B) how do you prepare the wet mount slide for light microscopes
- place a drop of water on the slide
- place your sample specimen in your drop of water
- set a cover slip over the sample specimen at a 45 degree angle
B) how do you prepare the stained slides for light microscopes
- Set a cover slip over the sample specimen at a 45o angle.
-Place 1 drop stain on the other side of the cover slip.
-The staining agent will begin to seep under the cover slip as the paper towel draws water out from the other side.
B) what and how does an eye piece graticule work
- an eye piece graticule is a small transparent ruler used to see the size of the object you are looking at
B) what is a stage micrometre
a microscope slide with a slide divided scale marked on the surface used to calibrate the eye piece graticule
(c) what are stains
stains are coloured chemicals that bind to molecules which allows the different structures in specimens to become more visible
(C) what is meant by differential staining
using different stains which are used so different tissues are stained different colours
what are the conversions between cm, mm, micrometres, nanometres
cm -> mm x10
mm -> micrometre x1000
micrometre -> nanometres x1000