2.1 cell structure Flashcards
Name the 4 types of microscopes.
Optical microscope
Transmission electron microscope
Scanning electron microscope
Laser scanning confocal microscope
What is an optical microscope?
It produces a 2D image and visible light passes through the specimen.
What is a transmission electron microscope?
Electrons pass through a specimen to a detector. Produces a 2D image
What is a scanning electron microscope?
Specimen is coated in metal, electrons scan across surface and electrons bounce off to detectors. Produces a 3D image.
What is a laser scanning confocal microscope?
Laser scans across specimen but scans at different depths to view different layers. Produces a 3D image of interior.
Pros of optical microscopes.
Can view living organisms.
Cons of optical microscopes.
Lower resolution and magnification than electron microscope.
Pros of electron microscopes.
Higher resolution and magnification
Scanning electron microscope produces 3D images.
Cons of electron microscopes.
Thin specimen required
Can only view dead specimens
Complicated processing which can result in artefacts.
Pros of laser scanning confocal microscopes.
Can view living organisms
3D images
Higher resolution than optical microscope
Cons of laser scanning confocal microscopes.
Lower resolution than electron microscopes.
What does resolution mean?
Smallest distance apart where 2 objects are seen as separate.
How can you increase contrast?
By adding a stain. (differential stain)
What is the magnification equation?
Image size = actual size x magnification
What is a graticule?
A ruler which is fitted into the eyepiece of a microscope.
Why do we use graticules?
Used to measure specimens.
What is a stage micrometer?
Microscopic ruler on a microscope slide.