2 Intro To Urinalysis Flashcards
the beginning of laboratory medicine.
Analyzing urine
Analyzing urine was the beginning of
Laboratory medicine
References to the study of urine can be found in (2)
drawings of cavemen
Egyptian hieroglyphics
Basic observation of urine
color
turbidity
odor
volume
viscosity
sweetness
He wrote a book on “uroscopy.”
Hippocrates
Hippocrates wrote a book on
Uroscopy
Year when Hippocrates wrote a book on uroscopy
5th Century BC
This had been developed describing the significance of 20 different color of urine
Color charts
color charts had been developed that described the significance of _____
20 different colors
Year when color charts was developed
1140 CE
He discovered albuminuria by boiling urine
Frederick Dekkers
Frederick Dekkers discovered ____
Albuminuria
How did Frederick Dekkers discovered albuminuria
He boiled the urine
He developed methods of quantitating microscopic sediments
Thomas Addis
Year when Thomas Addis developed quantitative methods on microscopic sediments
1925
Thomas Addis developed methods of quantitating what
Microscopic sediments
test used to diagnose kidney and urologic diseases by quantitatively counting red blood cells, white blood cells, epithelial cells, and circumferences using a 12 hour urine
Addis count
Addis count uses what type of spx
12 hour urine
He introduced the concept of urinalysis as part of a doctor’s routine px examination
Robert Bright
He wrote a book inspiring the england people to pass the first medical licensure law
Thomas Bryant
Daily urine output
1200 mL
Ultrafiltrate of plasma
Urine
Composition of the urine
95% water
5% solute
Organic components found in the urine
Urea, creatinine, uric acid
Inorganic components found in urine
Chloride
Sodium
Potassium
Phosphate
Ammonium
Calcium
Major inorganic component found in urine
Chloride
Cells, casts, crystals, mucus, bacterias are known as
Formed elements
This depends on the amount of water the kidney excrete
Urine volume
Urine volume depends on the amount of ____ the kidney excrete
Water
Average range of urine output
600-2000 mL
Urine volume is affected by
Fluid intake
Fluid loss from non renal sources
ADH secretion
Increased amount of dissolved solids
Decrease in urine output
Oliguria
Oliguria
____ in children
____ in adults
< 0.5 mL/kg/hr in children
<400 mL in adults
Cessation of urine flow
Anuria
Increase in the nocturnal excretion of urine
Nocturia
Increase in daily urine volume
Polyuria
Polyuria
____ in children
____ in adult
> 2.5 to 3mL/kg/day in children
2.5L/day in adult
What type of diabetes
Low specific gravity
Diabetes insipidus
Frequent fluid intake
Polydipsia
Recommended capacity of urine container
50 mL
Volume of spx used for analysis
12 mL
Urine container that contains a needle with a straw attachment
BD vacutainer urine collection device
If there will be a delay, the spx should be refrigerated at ___ C
2-8 C
Ways to handle a spx if there will be a delay with testing
Ref
Chemical preservatives
Urine preservative
Prevents bacterial growth
Refrigeration
Disadvantage of refrigerating spx
Precipitation of amorphous phosphates and urates
Best preservative for cultured spx
Boric acid / acid
Urine preservative
Excellent sediment preservative
Formalin
Urine preservative
Used for formed elements
Formalin
Urine preservative
Acts as a reducing agent
Formalin
Urine preservative
Good for drug analysis
Sodium fluoride
Urine preservative
Inhibits reagent strip test for glucose, blood, and leukocyte
Sodium fluoride
Sodium fluoride inhibits reagent strip test for
Glucose, blood, leukocyte
Urine preservative
Convenient when refrigeration is not possible
Commercial preservative tablet
Color is modified or darkened due to
Oxidation or reduction of metabolites
Analyte that has been modified or darkened due to oxidation or reduction of metabolites
Color
This is decreased due to bacterial growth and precipitation of amorphous material
Clarity
Increased ammonia smell is due to
Bacterial multiplication of urease positive bacteria
pH is ___ thru time
Increased
This analyte is decreased due to volatilization and bacterial metabolism
Ketones
First morning urine is also known as
8 hour urine sample
Ideal screening spx
First morning spx
First morning spx is acidic or basic in nature
Acidic
Spx used for evaluating orthostatic proteinuria
First morning spx
Principle of timed spx
Begin and end w/ an empty bladder
Timed spx for creatinine
24 hour urine
What spx is used for addis count
12 hour urine
Spx used to test urea
2 hour urine
Spx used to test amylase
2 hour urine AM
Spx used to test urobilinogen
2 hour urine PM
Spx used to test nitrite
4 hour urine
Analyte tested using 24 hour urine
Creatinine
What is tested using 12 hour urine
Addis count
Analyte tested using 2 hour morning urine
Amylase
Analyte tested using 2 hour PM urine
Urobilinogen
Analyte tested using 4 hour urine
Nitrite
It is the most vulnerable part of drug testing
Urine collection
Normal color of urine
Yellow