2. Innate immune defences & inflammation Flashcards
What is innate immunity?
1st line of defence against infection
Present at birth and passed down genetically
Occurs within minutes of pathogen recognition
Soluble innate immune molecules
Enzymes such as Lysozyme – disrupts bacterial cell walls; found in blood and tears
Antimicrobial peptides - disrupt microbial membranes
Collectins, ficolins and pentraxins –bind to pathogens targeting them for phagocytosis and activate complement
Complement components - lyse bacteria, opsonise bacteria, induce inflammation
Enzymes e.g. lysozyme
disrupts bacterial cell walls; found in blood and tears
Antimicrobial peptides
disrupt microbial membranes
Collectins, ficolins and pentraxins
bind to pathogens targeting them for phagocytosis and activate complement
Complement components
lyse bacteria, opsonise bacteria, induce inflammation
Lysozyme
Lysozyme disrupts peptidoglycan
Lysozyme is secreted by phagocytes and paneth cells from the small intestine
Cleaves the bond between the alternating sugars that make up peptidoglycan
Most effective against Gram-positive bacteria
Histatins
Produced in the oral cavity. Active against pathogenic fungi, e.g. Candida albicans
Cathelicidins
LL-37 broad-spectrum antimicrobial activity against both Gram-negative and Gram-positive bacteria
Antimicrobial peptides
histatins, defensins, cathethelicidins
Cover epithelial surfaces, found in saliva
Constitutively secreted by neutrophils, epithelial cells and paneth cells in the crypts of the small intestine
Kill bacteria in minutes, by disrupting the membrane
Also attack fungi, viruses (influenza and herpes virus)
Inhibit DNA and RNA synthesis
Defensins
Two classes – α, β defensins
35-40 aa amphipathic peptides which means they have both hydrophilic and hydrophobic regions on their cell surface.
Disulphide bonds stabilise the structure to have a positively charged region separated from a hydrophobic region
Disrupt microbial membranes but not that of the host
Collectins
Collectins have globular lectin like heads that bind bacterial cell surface sugars. Sialic acid hides mannose antigens on host cells
Ficolins
Ficolins recognise acylated compounds (COCH3) such as n-acetylglucosamine, a monosaccharide found in bacterial cell walls
Pentraxins
Pentraxins are cyclic multimeric proteins found in the plasma
C-reactive protein (CRP) is used as a clinical measure of inflammation
Actions of collectins, ficolins and pentraxins
Soluble pattern recognition receptors
Act as opsonins that bind to pathogens and infected cells targeting them for phagocytosis
Activate complement through the classical pathway/lectin pathway
Complement system
Series of over 30 proteins that constantly circulate in blood and fluids that bathe the body tissues
When they detect presence of foreign material, they initiate a cascade of reactions that amplify the signal
When activated, cooperate with other host defence systems to generate inflammation and rapidly remove the pathogen
Most made by the liver but also produced by monocytes, macrophages and epithelial cells of the intestine and urinary tract
Complement components
Circulate as a pro-form (inactive) in the blood
Numbered in the order they were discovered, not in the order they are activated
Some have proteolytic enzymatic activity
On activation they split into a small and large fragments triggering an amplification cascade
Normally ‘a’ is the small fragment - except c2a
Effects mediated by complement components
Lysis opsonisation activation of inflammatory response Clearance of immune response membrane attack complex complement receptor activation
Classical pathway
Initiated by C1 activation
C1 is a complex of three proteins: C1q, C1r and C1s
The structure of C1 is dominated by C1q
a large molecule of 18 polypeptides that form six collagen like triple helix structures
Classical pathway: activation
Triggered when C1 binds to the Fc region of an antibody – antigen complex
C1 must bind at least 2 FC domains
IgM is the most efficient at activating complement as it has 5 Fc domains. IgG1 and IgG3, and to a lesser extent IgG2 can also activate complement when close together bound to antigen
How does IgM bind C1q?
Serum IgM cannot bind C1 as it has a planar conformation, the shape changes on binding antigen to reveal binding sites for C1q
Classical pathway: amplification
Binding C1q with the Fc domain causes a conformational change in C1r C1s is cleaved and can activate C2 and C4 splitting into their large and small fragments C3 convertase (C4b2a) can then activate over 200 C3 molecules producing a massive amplification of the signal C4b, C2a and C3b fragments form the C5 convertase that activates C5 leading to the membrane attack complex
Lectin pathway
Antibody independent, activated by ficolins and mannose binding lectin (MBL)
MBL binds mannose residues on carbohydrates and glycoproteins on bacteria and some viruses
Similar downstream mechanism to the classical pathway
Upon binding MBL forms a complex with MASP-1 and MASP-2 (serine proteases)
Active complex cleaves C2 and C4
Alternative pathway
C3 spontaneous hydrolyses into C3a and C3b
C3b binds to a cell membrane and factor B, making it susceptible to cleavage by factor D to Bb
C3bBb has a half-life of 5 min, unless it binds the serum protein properdin, which extends it half-life to 30 min by protecting it from proteases
C3b,Bb can hydrolyse more C3 creating more C3b which can amplify the signal
Membrane attack complex
MAC forms a pore that inserts into the membrane allowing diffusion of ions and small molecules, water moves into the cell killing it
Human cells have soluble and cell surface associated proteins that prevent MAC formation
Hereditary angioedema: C1 inhibitor deficiency
Classical complement cascade easily activated but can be treated with an injection of C1 inhibitor
Complement deficiency
Patients deficient of components of the complement pathway experience recurrent infections
MBL deficiency causes serious pyogenic infections in neonates and children
C3 deficiency is the most severe leading to successive severe infections
Patients deficient of C8 are prone to infection with Neisseria meningitis
Complement deficiency in SLE
90% of people deficient for C4 develop the autoimmune disease systemic lupus erythematosus (SLE)
C4 deficiency means less C3b (C4b2a is C3 convertase)
C3b bound to immune complexes binds to CR1 on erythrocytes which transports them to phagocytes in the liver and spleen.
Phagocytes recognise the immune complexes via their Fc receptors and engulf them
