2 component regulatory systems and phosphorelay systems

Question 1

OmpF

Answer 1

porin that has large pores allowing solutes to diffuse across the OM more rapidly. it is used when the Aw is high like in a pond or stream

Question 2

OmpC

Answer 2

a porin that has small pores and is used when Aw is lower. exclude large toxic molecules like bile salts

Question 3

EnvZ

Answer 3

PM protein, a sensore kinase. when Aw decrease it autophosphorylates and then phosphorylates OmpR

Question 4

OmpR

Answer 4

a cytoplasmic portein. response regulator. when phosphorylate, OmpR activates transcription of ompC and represses transcription of omfF

Question 5

what activates micF

Answer 5

OmpR-P also activates expression of micF

Question 6

fn of micF

Answer 6

micF RNA is not translated, it acts as antisense RNA. anitisense RNA binds to 5’ end of exisitng ompF mRNA by complementary base pairing preventing translation by preventing 30S ribosomal subunit from binding to RBS, thus inhibits expression of ompF