2. Allelic diversity

Question 1

What is DNA polymorphism ?

Answer 1

indicates the allele variation at DNA level, it can be estimated using DNA markers.

Question 2

Define molecular marker…..

Answer 2

specific fragment/sequence of DNA that can be identified somewhere in the whole genome.

Question 3

What is a microsatellite

Answer 3

consists of a specific sequence of DNA bases or nucleotides which contain mono-, di-, tri-, or tetra tandem repeats. For example GTGTGTGTGT = (GT)5

Question 4

STR =

Answer 4

short tandem repeat

Question 5

What are flanking regions?

Answer 5

Regions surrounding the marker locus

Question 6

What are SNP’s

Answer 6

• Single base change in a DNA sequence
• Rich source of genetic variation
• Usually bi-allelic

Question 7

What are the practical considerations when it comes to DNA markers?

Answer 7

• Can it be reproduced under diffirent conditions
• how labour intensive is it
• cost
• what quantity of dna is required