#2

Question 0

TLR 4 Example

Step 2: ______ binds _____, activates _______ to phosphorylate ______ ==> leads to activation of _____

Answer 0

MyD88 binds TLR4
activates IRAK4 to phosphorylate TRAF6
leads to activation of IKK

Question 1

TLR 4 Example

Step 1: Complex of _____, _____, _____, & _____ assembled on surface

Answer 1

TLR4
MD2
CD14
LPS

Question 2

TLR4 Example

Step 3: _____ phosphorylates _____, leading to its degradation and the release of _____, which enters ________

Answer 2

IKK phosphorylates IkB (I kappa B)
release of NFkB (NF kappa B)
enters nucleus

Question 3

TLR4 Example

Step 4: _____ activates transcription of genes for _________ ______, synthesized in cytoplasm and secreted via _____

Answer 3

NFkB activates transcription of genes for Inflammatory Cytokines
secreted via ER

Question 4

Anaphylatoxins do what?

Answer 4

cause degranulation of mast cells/basophils and release vasoactive substances

Question 5

Acute-Phase Proteins

• -> ____ circulating ____ proteins
• -> induced _____ by _____ after _____
  ex. _____-binding _____ (activates C.C.)
  ex. _-_____ _____ (binds phosphorylcholine on microbes for phagocytosis)

Answer 5

blood circulating plasma proteins
induced rapidly by cytokines after infection

ex Mannose-binding lectin, activates complement cascade
ex C-Reactive Protein, phagocytosis

Question 6

Macrophages secrete everything EXCEPT:

Answer 6

IFN v

Interferon gamma

Question 7

Professional APCs (3)

Answer 7

1. Dendritic Cells (DCs)
2. Macrophages
3. B Cells

Question 8

In Spleen, each arteriole surrounded by

P.A.L.S.

Answer 8

Periarticular Lymphoid Sheath (PALS)

Question 9

PALS contain 2 zones:

Answer 9

B cell zone

T cell zone

Question 10

PALS attached to _______ containing a ______ ______

Answer 10

follicle containing a germinal center

Question 11

White Pulp is….

Answer 11

PALS + Lymphoid Follicles

Question 12

Th cells have CD4+ that directs them to

Answer 12

Class II MHC

Question 13

Cytotoxic T cells have CD8+ which directs them to

Answer 13

Class I MHC

Question 14

Class I MHC on ____ ________ _____

Presents to _______ ONLY

Answer 14

Class I MHC on all nucleated cells

Presents to CD8+ T cells

Question 15

Class II MHC is only on ____________ ___

Answer 15

professional APCs

Question 16

Antibody structure:

_______ of 2 pair identical ______ and ______ _______

Answer 16

Tetramer of 2 pair identical heavy and light chains

Question 17

Antibody structure

Both chains have ________ and _______domains

Answer 17

variable and constant

Question 18

Antibody Structure
Variable Region (abbreviation) is for \_\_\_\_\_\_ \_\_\_\_\_\_\_

Answer 18

F(ab’)2

Ab recognition

Question 19

Antibody Structure
Constant region (abbreviation) is \_\_\_\_\_\_\_\_\_  \_\_\_\_\_\_\_\_

Answer 19

Fc

effector property

Question 20

Light Chains (greek letters) (2)

Answer 20

Kappa

Lambda

Question 21

Heavy Chains (greek letters) (5)

Answer 21

Gamma, alpha, mu, theta, delta

Question 22

Antibody 5 classes/isotypes

Which 2 have subclasses?

Answer 22

1. IgA** 1&2
2. IgD
3. IgE
4. IgG** 1,2,3,4
5. IgM