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Question 1

What are the main types of microscopes?

Answer 1

• compound light microscope
• stereo dissecting microscope
• scanning electron microscope
• transmission electron microscope

Question 2

What is the characteristic of the microscope that indicates the amount of enlargement of an image, for example, 100X?

Answer 2

magnification

Question 3

With a compound light microscope, the maximum useful magnification is about?

Answer 3

1000X

Question 4

The lens that is within the eyepiece of the light microscope is called the?

Answer 4

ocular

Question 5

The wheel under the stage that adjusts the amount of light is called the?

Answer 5

diaphragm

Question 6

To focus a specimen, it is best to start with which objective?

Answer 6

low power

Question 7

When using the high power objective, you should adjust the?

Answer 7

fine focus

Question 8

A microscope has a 4x ocular lens and a 10x objective, what is this microscope’s total magnification?

Answer 8

40x

Question 9

The scanning, low, and high power objectives are mounted on the?

Answer 9

revolving nosepiece

Question 10

What methods are used in histological examination?

Answer 10

• radiography
• microscopy of preparations made of organs and tissues

Question 11

What is not the object of histological study of organisms?

Answer 11

• various environmental factors
• genome of an organism

Question 12

What instrumental method is not used for microscopic examination of histological slides?

Answer 12

visual examination with the naked eye

Question 13

What is the correct sequence of steps in the preparation of a permanent histological preparation?

Answer 13

• the capture of material
• fixation
• processing
• embedding
• sectioning
• staining

Question 14

What does not characterize the process of tissue fixation?

Answer 14

increasing the volume of fixed structures due to their hydration

Question 15

The main mechanism of fixation is?

Answer 15

irreversible coagulation of tissue proteins

Question 16

What does not characterize the process of dehydration of tissue structures?

Answer 16

linking tissue structures with water molecules

Question 17

Using the method of ultracentrifugation, it is possible to divide the cell contents into the following components:

Answer 17

• organelles
• macromolecules

Question 18

What is the optimal thickness for paraffin histological sections?

Answer 18

5-10 µm

Question 19

Why is the term ‘basophilic’ used for hematoxylin-staining?

Answer 19

because it has the chemical properties of the base

Question 20

Why is the term ‘acidophilic’ used for eosin-staining?

Answer 20

because it has the chemical properties of acid

Question 21

What does the term ‘basophilic staining’ mean?

Answer 21

staining of cell and tissue structures with basic colorant

Question 22

What does the term ‘oxyphilic staining’ mean?

Answer 22

staining of cellular and tissue structures with acid colorant

Question 23

What does the term ‘neutrophilic staining’ mean?

Answer 23

staining of cellular and tissue structures with both acidic and basic colorant

Question 24

Which structural elements of the cells do not stain basophilic?

Answer 24

• nucleolus
• smooth endoplasmic reticulation

Question 25

The resulting magnification of a light microscope is?

Answer 25

multiplication of the magnifications of objective lens and eyepiece

Question 26

Acidophilic colorant eosin stains the cytoplasm in?

Answer 26

pink-yellow color