[1S] UNIT 1.3-4 Laboratory Mathematics & Instrumentation Flashcards
Different ways of expressing concentration of solutions
• Molarity
• Normality
• Molality
• Percent solutions
• X solution
is defined as the number of units of solute to the number of units of solvent.
Ratio
T/F: Dilution is always expressed as a colon (x:x).
F; Ratio
Solute:solvent
Ratio
is defined as the number of units of solute to the number of units of solution.
Dilution
To express dilution, a _______ _________ is used, which is the ratio of the concentrated material to the final concentration or volume of solution (Bishop et al., 2013).
Dilution Factor
Solute:solution
Dilution
Solute:solution
Dilution
T/F: Dilution is done when:
• Sample is limited
• Need to establish titer
• Standard curve is needed
T
For sterilization of materials and supplies needed in the laboratory; also for disinfecting infectious waste (121C, 15 psi, for 20 minutes)
Autoclave
temp, psi, minutes using autoclave
121C, 15 psi, 20 mins
To evaporate to dryness materials after washing and/or after autoclaving (30 - 300 C)
Drying Oven
For weighing reagents/materials
Analytical Balance
For melting agarose powder in buffer
Microwave
For amplification of nucleic acids; commonly known as PCR machine
Thermal Cycler