[1S] UNIT 1.3-4 Laboratory Mathematics & Instrumentation Flashcards
Different ways of expressing concentration of solutions
• Molarity
• Normality
• Molality
• Percent solutions
• X solution
is defined as the number of units of solute to the number of units of solvent.
Ratio
T/F: Dilution is always expressed as a colon (x:x).
F; Ratio
Solute:solvent
Ratio
is defined as the number of units of solute to the number of units of solution.
Dilution
To express dilution, a _______ _________ is used, which is the ratio of the concentrated material to the final concentration or volume of solution (Bishop et al., 2013).
Dilution Factor
Solute:solution
Dilution
Solute:solution
Dilution
T/F: Dilution is done when:
• Sample is limited
• Need to establish titer
• Standard curve is needed
T
For sterilization of materials and supplies needed in the laboratory; also for disinfecting infectious waste (121C, 15 psi, for 20 minutes)
Autoclave
temp, psi, minutes using autoclave
121C, 15 psi, 20 mins
To evaporate to dryness materials after washing and/or after autoclaving (30 - 300 C)
Drying Oven
For weighing reagents/materials
Analytical Balance
For melting agarose powder in buffer
Microwave
For amplification of nucleic acids; commonly known as PCR machine
Thermal Cycler
A dry place to heat solution due for incubation
Heat Block
Used to separate solid particles from liquid suspension through centrifugal force
Centrifuge Machine
Used to read absorbances of solution as light passes through the sample
Absorbance Reader
For visualizing electrophoretic bands
Gel Documentation Viewer
A working area designed to protect the workers from infectious agents;, at the same time, protects the materials being manipulated from environmental contaminants
Biosafety Cabinet