17 Transcription and Translation

Question 0

Strand not copied

Answer 0

Antisense strand, non-template strand, coding strand

Question 1

Transcription

Answer 1

Synthesis of mRNA version of instruction

- done by RNA polymerase : transcribes on strand (sense)

Question 2

Difference between sequence of DNA and mRNA

Answer 2

Same sequence except U instead of T

Question 3

Does RNA polymerase require primer?

Answer 3

No

Question 4

Initiation

Answer 4

First phase of transcription

-sigma protein subunit binds to mRNA

Question 5

Holoenzyme

Answer 5

Sigma bound RNA polymerase

Question 6

Sigma allows

Answer 6

RNA polymerase to read receptor

Question 7

Bacterial promoters

Answer 7

Are comprise of 40-50 base pairs
Have to regions = -10 box and -35 box

Tell sigma where to start

Question 8

-35

Answer 8

TTGACA

Question 9

Transcription begins

Answer 9

When sigma in holoenzyme binds to boxes

Question 10

Sigma does

Answer 10

Opens DNA helix

• the template strand threaded through polymerase
• once RNA polymerization begins sigma dissociates

Question 11

Elongation

Answer 11

RNA polymerase moves along DNA template

Question 12

Termination

Answer 12

RNA polymerization transcribes a transcription termination sequence

Question 13

Bacteria transcription

Answer 13

Hairpin

Question 14

Difference between eukaryote prokaryote

Answer 14

TATA box

Question 15

Eukaryote

Answer 15

No sigma
Basal transcription factor
RNA polymerase does not bind directly to promoter

Question 16

Basal trans

Answer 16

Many proteins

Not part of holo

Question 17

Bacteria vs eukary

Answer 17

Bac: DNA converted directly to mRNA
Euk: product of trans is immature - not complet

Question 18

The protein coding regions

Answer 18

Contain non-coding regions
They must but cut out
-introns