17 Transcription and Translation Flashcards

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0
Q

Strand not copied

A

Antisense strand, non-template strand, coding strand

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1
Q

Transcription

A

Synthesis of mRNA version of instruction

- done by RNA polymerase : transcribes on strand (sense)

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2
Q

Difference between sequence of DNA and mRNA

A

Same sequence except U instead of T

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3
Q

Does RNA polymerase require primer?

A

No

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4
Q

Initiation

A

First phase of transcription

-sigma protein subunit binds to mRNA

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5
Q

Holoenzyme

A

Sigma bound RNA polymerase

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6
Q

Sigma allows

A

RNA polymerase to read receptor

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7
Q

Bacterial promoters

A

Are comprise of 40-50 base pairs
Have to regions = -10 box and -35 box

Tell sigma where to start

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8
Q

-35

A

TTGACA

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9
Q

Transcription begins

A

When sigma in holoenzyme binds to boxes

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10
Q

Sigma does

A

Opens DNA helix

  • the template strand threaded through polymerase
  • once RNA polymerization begins sigma dissociates
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11
Q

Elongation

A

RNA polymerase moves along DNA template

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12
Q

Termination

A

RNA polymerization transcribes a transcription termination sequence

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13
Q

Bacteria transcription

A

Hairpin

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14
Q

Difference between eukaryote prokaryote

A

TATA box

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15
Q

Eukaryote

A

No sigma
Basal transcription factor
RNA polymerase does not bind directly to promoter

16
Q

Basal trans

A

Many proteins

Not part of holo

17
Q

Bacteria vs eukary

A

Bac: DNA converted directly to mRNA
Euk: product of trans is immature - not complet

18
Q

The protein coding regions

A

Contain non-coding regions
They must but cut out
-introns