1.6 Proteins Flashcards
What is the structure of an Amino Acid
Amino group ( NH2)
Carboxyl groups ( COOH)
Hydrogen atom
R grouped
How does a peptide Bond form
When amino acid monomers join together to form a di-peptide during a condensation reaction.
The two amino and then become linked by a new peptide bond between the carbon atom and in of amino acid and the nitrogen atom the other.
Secondary structure of a protein
The amino acid form weak bonds , hydrogen bond to form a long polypeptide chain
Tertiary structure of proteins
Secondary structure is folded further different bonds are formed
Disulfide bridges
Ionic bonds( friend between any carboxyl and amino groups not involved in forming peptide bonds) weaker than disulfide bonds so easily broken by pH changes
Hydrogen bonds - numerous but easily broken
What is the test for proteins
Place a sample of solution in a fat tube add an equal volume of NaOH ( sodium hydroxide) solution at Rtp
Add a few drops of dilute copper sulfate solution., mix gently
Purple coloration indicated a positive presence of peptide bond as it detects the peptide bonds, if no presence it remains blue
What are the two basic types of proteins
Fibrous proteins- have structural functions
Globular proteins- carry out metabolic functions