1.5 Research Methods

Question 1

the study of the composition of body tissue

Answer 1

Histology

Question 2

a tissue stain that fills all cell bodies, to help measure the size and number of cell bodies in a region

Answer 2

Nissl Stain

Question 3

a tissue stain that fills a small proportion of neurons, to help identify the type and precise shapes of the neurons in a region

Answer 3

Gogli Stain

Question 4

uses radioactive source that shows the distribution of radioactive chemicals in tissue

Answer 4

Autoradiography

Question 5

create antibodies against a protein of interest, to visualize the distribution of a particular protein in tissue.

Answer 5

Immunohistochemistry

Question 6

Uses a nucleotide prone that is complementary to the transcript of interest to detect particular RNA transcript. Labels on those neurons in which a gene of interest has been turned on.

Answer 6

In situ hybridization

Question 7

A type of histological stain that is taken up by neurons and transported over the routes of their axons, allowing the sources and targets of axons to be visualized

Answer 7

Tract tracers

Question 8

Medium-resolution images used to examine brain structure through computer analysis of X-ray absorption at several position around the head

Answer 8

CAT or CT scan (computerized axial tomography)

Question 9

Uses magnetism and radio0frequency energy to create images of the gross structure of a living brain.

Answer 9

MRI (Magnetic Resonance Imaging)

Question 10

Modified form of MRI in which the diffusion of water in a confine space is exploited to produce image of axon fiber tracts, and learn how networks of brain structure work together.

Answer 10

DTI (Diffusion tensor imaging)

Question 11

Magnetic resonance imaging that detects change in blood flow and therefore identifies regions of the brain that are particularly active during a given task. To image localized change in the brain’s activity.

Answer 11

fMRI (functional magnetic resonance imaging)

Question 12

Tracks the metabolism of injected radioactive substance in the brain, in order to map brain activity

Answer 12

PET (Positron Emission Tomography)

Question 13

Examine brain function through applying strong magnetic fields to stimulate cortical neurons in order to identify discrete areas of the brain that are particularly active during specific behaviors.

Answer 13

TMS (transcranial magnetic stimulation)

Question 14

creates maps of brain activity during cognitive tasks by measuring tiny magnetic fields produced by active neurons

Answer 14

MEG (magnetoencephalorgraphy)