1.4 - Proteins Flashcards
What is the general structure of an amino acid?
- COOH carboxylic acid group
- NH2 amine group
- R variable region consisting of carbon chains & other functional groups
Describe how to test for proteins in a sample.
Use the Biuret test which confirms the presence of a peptide bond.
1) To make test solution alkaline, add a few drops of sodium hydroxide solution.
2) Add copper (II) sulfate solution.
Negative result: solution remains blue
Positive result: solution changes to purple
How many amino acids are there and how do they differ from one another?
20
Only differ in R group
How do dipeptides and polypeptides form?
Condensation reaction forms peptide bonds whilst releasing a water molecule.
Dipeptide = 2 amino acids
Polypeptide = 3 +
How do dipeptides and polypeptides form?
Condensation reaction forms peptide bonds whilst releasing a water molecule.
Dipeptide = 2 amino acids
Polypeptide = 3 +
What are the levels of protein structure?
Primary, secondary, tertiary & quaternary
Define ‘primary structure’ of a protein.
Primary structure is the sequence, number & type of amino acids in the polypeptide chain.
Determined by sequence of codons on mRNA
Define ‘secondary structure’ of a protein.
Hydrogen bonds form between amino acids in the polypeptide chain, automatically making it coil into an alpha helix, or fold into a beta pleated sheet.
Define ‘tertiary structure’ of a protein.
Hydrogen & ionic bonds, and disulfide bridges form between parts of the polypeptide chain.
Describe disulfide bridges in the tertiary structure of proteins.
Strong covalent S-S bonds between molecules of the amino acid cysteine
Describe ionic bonds in the tertiary structure of proteins
Relatively strong bonds between charged R groups.
pH changes causes ionic bonds to break.
Describe hydrogen bonds in the tertiary structure of proteins.
Numerous & easily broken.
Define ‘quaternary structure’ of a protein.
Some proteins are made of several polypeptide chains held together by bonds. The quaternary structure is the protein’s final 3D structure.
Describe the structure of globular proteins.
- Spherical & compact
- Hydrophilic R groups face outwards & hydrophobic R groups face inwards, so usually water soluble
Describe the function of globular proteins.
Involved in metabolic processes (e.g. enzymes & haemoglobin)