1.3 - Microscopes Flashcards
______ are instruments designed to produce ______ visual or photographic images of small objects
Microscopes
Magnified
The ______ must accomplish 3 tasks:
- Produce a ______ image of the specimen
- Separate the ______ in the image
- Render the ______ ______ to the ______ or camera
Microscope Magnified Details Details visible Human eye
Microscopes increase ______ - the minimum distance two points can be apart and still be distinguished as two separate points
Resolution
A microscope has one or more ______ that make an enlarged image of an object
Lenses
______ microscopes are used to view objects and are illuminated by visible ______
Light x2
______ microscopes use only one ______ and are similar to a magnifying glass 🔍
Simple
Lens
______ microscopes use ______ or more lens placed one on top of the other. It commonly has one lens in the eyepiece and one in each OBJECTIVE lens. Can magnify as much as 2000X.
Compound
Two
______ microscopes give a ______ view of an object and are used for ______
Stereoscopic
3-D
Dissections
______ microscopes illuminate specimens with a beam of ______ instead of a beam of light. Can magnify us to 1.2 mln times
Electron
Electrons
Electron ______ are the photographs of the image produced by an ______ microscope
Micrographs
Electron
Electron microscopes can achieve ______ images
3-D
______ electron microscopes:
Specimens are ______ sliced then placed under a vacuum to remove moisture and particles. Electrons are then transmitted through the specimens to view ______ internal structures and details.
Transmission (TEM)
Thinly
2-D
______ electron microscopes use a beam of ______ that sweeps over an object to create a ______ image (only the surface can be detected)
Scanning (SEM)
Electrons
3-D
______ laser ______ microscopes have a ______ beam directed at numerous planes created a series of ______ images or “optical ______”
Computer software then “______” the images together
Confocal Scanning (CLSM) Laser 2-D Slices Stiches
When specimens are difficult to see due to their pale colour or transparency the specimen can be ______ and it will attach to different parts of the specimen.
*disadvantage: the specimen is killed in the process
Stained
