1.2 cell structure and organisation Flashcards
which microscope has a greater magnification and higher resolving power?
electron microscope because it uses electrons (rather than light), which have a much shorter wavelength
(so electron microscopes can see many more structures called organelles in eukaryotic cells)
organelle definition
a specialised structure with a specific function inside a (eukaryotic) cell
eukaryotic cells (eukaryotes) definition
they contain DNA in chromosomes in a nucleus, and possess membrane-bound organelles e.g plants and animals
resolving power definition
the minimum distance by which two points must be separated in order for them to be seen as two distinct points rather than a single focused image
what surrounds organelles?
and what are the advantages of this?
a membrane
potentially harmful chemicals, such as enzymes, are isolated, and molecules with particular functions, such as chlorophyll, can be concentrated in one area
what do membranes provide for organelles?
a large surface area for the transport of molecules and attachment of enzymes
what are the different units of measurements in biology? and what are they used to measure?
- metre (m) = used to measure larger organisms
- millimetre (mm) = used to measure tissues and organs
- micrometre = cells and organelles (10^-6m)
- nanometre (nm) = molecules (10^-9m)
what is the most convenient unit for measuring cells and organelles?
micrometres
what is the nucleus?
- the largest organelle of the cell, bounded by a double membrane
- present in the cytoplasm of a eukaryotic cell
- CONTAINS DNA WHICH CODES FOR PROTEIN SYNTHESIS
what are the different components in a nucleus?
- nucleolus
- chromatin
- nucleoplasm
- (nuclear) pore
- nuclear envelope
- endoplasmic reticulum
- ribosome
what is the nucleolus?
- a small spherical body found within the nucleus
- responsible for the production of rRNA and ribosomes
what is the nucleoplasm?
- like the cytoplams of a cell, but for a nucleus
- contains chromatin (which condenses to form chromosomes during cell division)
what is the nuclear envelope?
- the double membrane which encloses the genetic material
- has pores (to allow movement)
what are nuclear pores?
- gaps in the nuclear envelope that allow movement
- allows the passage of large molecules, such as mRNA and ribosomes
- allows transport of messenger/mRNA/nucleotides/ribosomes
where is the endoplasmic reticulum in the nucleus?
the outer membrane of the double membrane is continuous with the endoplasmic reticulum
what is chromatin?
- made of coils of DNA bound to protein (DNA-protein complex) but uncoils (is linear) when not during cell division
- condenses to form chromosomes during cell division
what are the main functions of the nucleus?
- enable protein synthesis (by creating ribosomes), cell division, growth and differentiation into specialised cells
- stores genetic material in the form of chromatin
- exchange of RNA with the rest of the cell
what is the endoplasmic reticulum?
- a network of membranes connected to the nucleus of the cell by the nuclear envelope
- has two types: rough and smooth
- main role is concerned with the transport of material through the cell
- a continuous membrane with many folds
- form interconnected flattened fluid-filled sacs called cisternae
what is the smooth endoplasmic reticulum?
- more tubular (tubular membrane)
- lacks ribosomes
- involved with the synthesis and transport of lipids
what is the rough endoplasmic reticulum?
- has ribosomes on the outer surface
- transports the proteins once they’ve been synthesised at the ribosomes (via the cisternae)
packages and transports proteins
what are cisternae?
interconnected flattened fluid-filled sacs in the endoplasmic reticulum
vesicle definition
a ‘bubble’ that can contain and transport molecules inside of it
what is the golgi body?
- stack of curves cisternae
- vesicles containing polypeptides (building blocks of protein) pinch off from the rough endoplasmic reticulum, and fuse with the golgi
- proteins are modified and packed into vesicles by the golgi body for export
- also involved in the transport and storage of lipids, and the production of glycoproteins and lysosome
what are the components of a mitochondria?
- circular DNA
- matrix
- outer membrane
- inter-membrane space
- inner membrane
- crista (cristae)
- 70s ribosomes
what is the usual size and shape of a nucleus?
usually spherical
and 10-20 micrometres in diameter
what is the usual size and shape of a mitochondria?
often cylindrical
1-10 micrometers in length
what type of cells have extensive endoplasmic reticulum?
cells that store large quantities of carbohydrates, proteins and fats, including liver and secretory cells
why type of cells contain lots of mitochondria?
they are present in all cells, but are found in much higher number in metabolically active cells e.g in muscle cells, that need a plentiful supply of ATP
what is the mitochondria?
- the site of aerobic respiration/ ATP production (energy)
- RELEASES energy
- has a double membrane
- being cylindrical, has a larger surface area than a sphere of the same volume (big surface area to volume ratio) + reduces the diffusion distance b/w the edge and the centre, making aerobic respiration more efficient
what is the cristae?
why is this useful?
- the inner membrane of the mitochondria is folded into structures called cristae
- it PROVIDES A LARGE SURFACE AREA for the attachment of enzymes involved in respiration
what is the inter-membrane space?
- the space in between the two membranes (outer membrane and the inner membrane) in a mitochondria
- fluid-filled
what is the matrix?
- the volume enclosed by the inner membrane in a mitochondria
- a solution containing many compounds, including lipids and proteins, 70s ribosomes and a small circle of DNA
- contains the enzymes we need for respiration
what are the 70s ribosomes and the small circle of DNA for in a mitochondria?
they enable mitochondria to make some of their own proteins and self-replicate
in a mitochondria, where do the reactions occur?
some occur in the matrix and others on the inner membrane
what are the components of a chloroplast?
- double membrane
- thylakoid
- thylakoid membrane
- inter-granal lamella
- granum
- 70s ribosomes
- starch grain
- stroma
- circular DNA
what are the chloroplasts?
- the site of photoynthesis
- absorbs sunlights and converts into sugar molecules
- found in photosynthetic plants
- surrounded by two membranes, comprising the chloroplast envelope
what is the thylakoid?
- a flattened membrane
- site of light absorption in the chloroplasts
- stacks of thylakoid contain the photosynthetic pigments, which include chlorophyll
what is the granum?
- stacks of thylakoids (between two and a hundred parallel stacks) in a chloroplast
- this arrangement produces a large surface area, efficient for trapping light energy
what is the lamella?
- they connect the stacks of thylakoids together and allow transport between the stacks in a chloroplast
what is the stroma?
- fluid-filled
- got enzymes used in photosynthesis
- filled with starch grains, 70s ribosomes and a circle of DNA
- like the cytoplasm/nucleoplasm but in a chloroplast
in chloroplasts, what do the 70s ribosomes and circular DNA enable them to do?
enable them to make some of their own proteins and self-replicate
(like mitochondria)
in chloroplasts, is the inner membrane folded?
no
(only in mitochondria)
why are thylakoids arranged how they are in chloroplasts?
it produces a large surface area so is efficient for trapping light energy
prokaryotes definition
a single-celled organism lacking membrane-bound organelles, such as a nucleus, with its DNA free in the cytoplasm
what are the components of a ribosome (when drawing a diagram)?
- 2 tRNA attachment sites
- mRNA attachment site
- large subunit
- small subunit
what are ribosomes made from? and where are they found?
- made from rRNA and protein
- found within the cytoplasm
are ribosomes surrounded by a membrane?
no
what is the role of ribosomes?
- the assembly of proteins during translation
- they are important in protein synthesis, as they are the site of translation, where mRNA is used to assemble the polypeptide chain
what is the size of ribosomes in prokaryotic cells? in eukaryotic cells?
- prokaryotic cells = 70s in size
- in the cytoplasm of eukaryotic cells = 80s, where they occur singly or attached to membranes on the RER (except 70s in mitochondria and chloroplasts)
what does the small subunit contain? the large subunit? in a ribosome
- the small subunit contains a mRNA attachment site
- the large subunit contains two tRNA attachment sites
how do ribosomes compare in size between nucleus and mitochondria?
ribosomes are much smaller than the nucleus and mitochondria
what is the endosymbiotic theory?
the theory that describes the origin of chloroplasts and mitochondria
- it has been suggested that these organelles arose from a symbiotic relationship between early cells that engulfed photosynthetic and respiratory bacteria over 1.5 billion years ago
- they both have 70s ribosomes and circular DNA (which are the type found in prokaryotes including bacteria)
what are lysosomes? what is their role?
- vesicles with (hydrolytic/digestive) enzymes inside to break down waste product
- their role is to digest worn out organelles within the cell, and foreign material that has been engulfed by phagocytosis e.g bacteria engulfed by a white blood cell
(also contain digestive enzymes that break down/recycle worn out organelles or cells)
how are lysosomes formed?
formed by being pinched off from the golgi body
what enzyme do lysosomes contain?
the digestive enzyme lysozyme
what are centrioles?
- present in all animal cells and most protoctistans (but not in the cells of higher plants)
- consist of two rings of microtubules (making cylinders) positioned at right angles to each other
- they organise the microtubules that make the spindle during cell division
- located just outside the nucleus
what is the function of a vacuole?
- supporting soft plant tissues
- but also store chemicals such as glucose and amino acids in the cell sap
what are vacuoles?
- fluid-filled sac bounded by a single membrane, the tonoplast
- most are large and permanent
in plants, what is the cell wall mostly made from?
cellulose
what are the functions of the cell wall?
- STRENGTH = providing strength to the cell wall, which resists the expansion of the vacuole due to osmosis, and so the cell becomes turgid, supporting the plant
- TRANSPORT = transports water and dissolved molecules and ions through gaps in the cellulose fibres (known as the apoplast pathway - the main way that water crosses the plant root)
- COMMUNICATION BETWEEN CELLS = communication between cells via pores in the cell wall which allow strands of cytoplasm called plasmodesmata to pass. this allows water to pass via the symplast pathway (important in the transport of water through a plant)
what is plasmodesmata?
- strands of cytoplasm
- they pass through the pores in the cell wall
- plasmodesma occurs where there is no cellulose thickening between two cells
- they run from one cell to the next
(this network of cytoplasm in connected cells is called the symplast)
do all plant cells contain chloroplasts?
no - root cells for example, being underground, would have no use for them
are cell walls found in animal cells? plant cells?
animals cells - absent
plant cells - present = it surrounds the cell membrane
are chloroplasts found in animal cells? plant cells?
animal cells - absent
plant cells - present = in cells above ground
are plasmodesmata found in animal cells? plant cells?
animal cells - absent
plant cells - present
are vaculoes found in animal cells? plant cells?
animal cells - present = but small, temporary; scattered throughout cell
plant cells - present = large, permanent, central; filled with cell sap
are centrioles found in animal cells? plant cells?
animals cells - present
plant cells - absent
what energy store is found in animal cells? plant cells?
animal cells - glycogen
plant cells - starch
what does the nucleus contain and what does it do?
- contains chromosomes
- in which the DNA encodes proteins
what do the nuclear pores in the nuclear envelope allow?
it allow mRNA molecules, transcribed off the DNA, to leave the nucleus and attach ribosomes in the cytoplasm or on the rough ER
what do ribosomes contain?
rRNA, transcribed from DNA located at the nucleolus
where does protein synthesis occur? what does it produce?
- occurs on ribosomes
- produces proteins in their primary structure
where are polypeptides made? where are they then moved to?
- polypeptides made on the ribosomes are moved through the RER and are packaged into vesicles
- the vesicles bud off the RER and carry polypeptides to the Golgi body, where they are chemically modified and folded
what does the Golgi body produce? what may they be?
- produces vesicles containing newly synthesised proteins
- these may be lysosomes, containing digestive enzymes, used within the cell
- they may be secretory vesicles, which carry the proteins to the cell membrane for exocytosis
what do secretory vesicles do?
carry the proteins to the cell membrane for exocytosis
what do phospholipids and triglycerides move through? where do they go?
- they move through the smooth endoplasmic reticulum
- to various destinations in the cell
what have eukaryotic cells?
- fungi
- protoctista
- plants
- animals
are viruses made of cells?
no - they are not made of cells and they are not classified with living organisms
- they seem to exist at the interface between living and non-living systems
prokaryote definition
a single-celled organism lacking membrane-bound organelles, such ad a nucleus, with its DNA free in the cytoplasm
eukaryote definition
an organism containing cells that have membrane-bound organelles, with DNA in chromosomes within the nucleus
what is an example of a prokaryotic cell?
a bacterium
what is the major distinguishing feature of prokaryotic cells?
- they have no nucleus
- or any internal membrane
so unlike eukaryotic cells, they have no membrane-bound organelles
- also they are unicellular and rarely form multicellular structures
in some prokaryotes, what increases the surface area of the cell’s membrane?
the infolding of the cell membrane in a mesosome or photosynthetic lamellae
for each one, state whether it’s in all prokaryotes or only some prokaryotes?
- DNA molecule loose in cytoplasm
- peptidoglycan (murein) cell wall
- slime coat
- 70S ribosomes
- flagella (one, some or many)
- photosynthetic lamellae holding photosynthetic pigments
- cytoplasm
- cell membrane
- mesosomes - possible site of aerobic respiration
- plasmids
- DNA molecules loose in cytoplasm - ALL
- peptidoglycan cell wall - ALL
- slime coat - SOME
- 70S ribosomes - ALL
- flagella - SOME
- photosynthetic lamellae - SOME
- cytoplasm - ALL
- cell membrane - ALL
- mesosome - SOME
- plasmids - SOME
what is the difference between prokaryotes and eukaryotes?
prokaryotes:
- smaller
- no organelles
- DNA free in cytoplasm
- no nuclear envelope
- plasmids may be present
- peptidoglycan (murein) cell wall
- no chloroplasts (but may use photosynthetic lamella for photosynthesis)
- no mitochondria (but may use mesosome for respiration)
- 70s ribosomes, free in cytoplasm
eukaryotes
- larger
- membrane-bound organelles
- DNA combined with protein in chromosomes
- double membrane nuclear envelope
- plasmids absent
- cellulose cell wall in plants, chitin in fungi
- chloroplasts in plants and some protoctista
- mitochondria present
- mesosome absent
- 80s ribosomes, free in cytoplasm or attached to ER