11.2 Membrane Dynamics Flashcards
liquid-ordered (Lo) state
gel-like state in which all types of motion of individual molecules are strongly constrained
VDW- much stabilization energy
tightly packed
liquid-disordered (Ld) state
state in which individual hydrocarbon chains are in constant motion (lateral and rotational)
VDW not as stabilizing
more space to move
L0 to Ld transition can be caused by
heat which produces thermal motion of side chains
fatty acid composition affects
membrane fluidity
at physiological temperatures, long chain saturated fatty acids:
tend to pack into an Lo phase (more VDW)
at physiological temperatures, kinks in unsaturated fatty acids:
interfere with packing, favoring the Ld state (less VDW)
shorter-chain fatty acyl groups favor the
Ld state
less SA, less VDW
based on the amount of VDW, for packing:
increasing VDW favors ordered
decreasing VDW favors disordered
sterols have paradoxical effects on bilayer fluidity
they interact with phospholipids containing unsaturated fatty acyl chains, compacting them and constraining their motion (ordered)
they associate with sphingolipids and phospholipids having long, saturated fatty acyl chains, making the bilayer fluid (disordered)
transbilayer movement of lipids requires
catalysis
transbilayer (“flip flop”)
movement has a large, positive free-energy change
membrane proteins facilitate the
translocation of individual lipid molecules
flippases
catalyze the translocation of the amino-phospholipids phosphatidylethanolamine (PE) and phosphatidylserine (PS) from the extracellular to the cytoplasmic leaflet of the plasma membrane
consume ~1 ATP per molecule of phospholipid translocated
related to the P-type ATPases (active transporters)
floppases
move plasma membrane phospholipids and sterols from the cytoplasmic leaflet to the extracellular leaflet
are ATP-dependent
members of the ABC transporter family
each specializes in movement of specific lipids
scramblases
move any membrane phospholipid across the bilayer down its concentration gradient
not dependent on ATP; some require Calcium
lead to controlled randomization of the head-group composition on the two faces of the bilayer – toward equilibrium
uncatalyzed transbilayer (“flip flop”) diffusion
is very slow
uncatalyzed lateral diffusion is
very fast