1.1 The world of the microscope

Question 1

What are living things made out of?

Answer 1

Cells

Question 2

What does 1 kilometre equal to metres?

Answer 2

1km = 1000m

Question 3

What does 1 cm equal to millimetres?

Answer 3

1 cm = 10 mm

Question 4

What does 1 millimetres equal to micrometres?

Answer 4

1 mm = 1000 micrometres (um)

Question 5

What does 1 micrometres equal to nanometres?

Answer 5

1 um = 1000 nanometres (nm)

Question 6

What is nanometres written in standard form?

Answer 6

1 x 10(-9) m

Question 7

When was the first light microscope developed?

Answer 7

Mid – 17th century.

Question 8

What was the development first looked with the light microscope?

Answer 8

Widely used to look at cells

Question 9

What are light microscopes and how does it work?

Answer 9

Light microscopes use a beam of light to form an image of an object.

Question 10

What can the light microscope magnify to?

Answer 10

2000 times

Question 11

What is the advantage of using the light microscope?

Answer 11

Cheap, used almost anywhere and can magnify live specimens.

Question 12

What components are involved in the light microscope?

Answer 12

• Eyepiece
• Objective lens
• Stage
• Slide
• Light
• Fine focus
• Coarse focus

Question 13

What did the invention of the electron microscope in the 1930s allowed biologists?

Answer 13

Understand more about the subcellular structures inside cells.

Question 14

What was this instrument used for?

electron microscope

Answer 14

Usage beam of electrons to form a image and each can magnify objects to around 2 000 000 times.

Question 15

What does transmission microscope allows us?

Answer 15

• Gies 2D images
• High magnification
• High resolution

Question 16

What does the scanning electron microscope allows us?

Answer 16

• Dramatic 3D images

- Lower Magnification

Question 17

What is the conditions for the electron microscopes?

Answer 17

• Large
• Very Expensive
• Kept in special temperature , pressure and humidity controlled rooms

Question 18

How to calculate magnification of an object by the objective lens?

Answer 18

Magnification = Image size / Size of real object

Question 19

What is the resolution?

Answer 19

The ability to distinguish between two separate points and it is the resolving power of the microscope that affects how much detail is shown.

Question 20

What resolving power does a light microscope have?

Answer 20

200 nm

Question 21

What resolving power does a scanning electron microscope have?

Answer 21

10 nm

Question 22

What resolving power does a transmission electron microscope have?

Answer 22

0.2 ( approximately distance of two atoms in solid substance)