1.1 Lab Techniques

Question 1

Give 4 examples of hazards in the lab

Question 2

Explain the difference between a risk and a hazards

Question 3

Describe some control measures that may be put in place to reduce risk

Question 4

Accuracy

Question 5

Validity

Question 6

Reliability

Question 7

Describe the difference between a log and a linear dilution

Question 8

Give an example of both a log and a linear dilution

Question 9

What formula do you use for linear dilutions

Question 10

Explain how a standard curve may be produced

Question 11

Give a practical use of a standard curve

Question 12

Explain why a buffer may be used

Question 13

Explain why a colorimeter should be recalibrated between uses

Question 14

What should a calibration blank contain

Question 15

When is it appropriate to use absorbance when using a colorimeter?

Question 16

When would transmission be more appropriate when using a colorimeter?

Question 17

Explain how a centrifuge can be used to separate molecules?

Question 18

Explain how chromatography can be used to separate molecules?

Question 19

Explain how affinity chromatography can be used to separate molecules

Question 20

Explain how gel electrophoresis can be used to
separate molecules

Question 21

Describe the differences
between Native Gel Electrophoresis and SDS-PAGE.

Question 22

Explain how molecules can be separated using their isoelectric points.

Question 23

What are immunoassay techniques and
how do they work?

Question 24

What are monoclonal antibodies?

Question 25

What are reporter enzymes

Question 26

What do reporter enzymes do?

Question 27

Explain how reporter enzymes are used

Question 28

What are immunoassay techniques

Question 29

How do immunoassay techniques work