1.1-> 1.3 Carbohydrates Flashcards
Hydrogen bonding
electrons within a (polarized) molecule are not evenly distributed but tend to spend more time at one position- region is more negatively charged
negative region and positive region attract one another, so weak electrostatic bonds form between the two
Polymer
Long chain molecule made up of monomer sub-units joined together
Condensation reaction
Each time a new sub-unit is attached to a polymer, a molecule of water is formed
2 examples of condensation reaction
- polypeptide from amino acid
- polysaccharide starch from monosaccharide glucose
Hydrolysis
When water molecules are used to break bonds that link sub-units of a polymer, whereby splitting the molecule into its constituent parts
2 examples of hydrolysis
- polypeptides into amino acids
- starch into glucose
One mole
Contains same number of particles as there are in 12g of Carbon12 aroms
6.022 x 10^23
Molar solution
Contains one mole of solute in each litre of solution
Carbon atom unusual feature
readily form bonds with other carbon atoms
this allows a sequence of carbon atoms of various lengths to be built up
these form a backbone which other atoms can be attached
Carbohydrates
made up of carbon and water
Single monomer of carbohydrates
monosaccharide(sugar) - soluble sweet-tasting substances
Monosaccharide basic formula
general formula (CH2O)n
Formula of glucose
C6H12O6
examples of monosaccharide
glucose
fructose
galactose
Test for reducing sugars
- Add 2cm3 of food sample to be tested into a test tube
- Add an equal volume of blue Benedict’s reagent(copper II sulphate)
- Heat the mixture in a gently boiling water bath for 5 minutes
- If reducing sugar is present, it forms an insoluble red/orange-brown precipitate of copper I oxide
Reducing sugar
sugar that can donate electrons to another chemical
e.g glucose, galactose and fructose
Disaccharide
When two monosaccharides are combined in a pair
3 examples of disaccharides
- glucose + glucose -> maltose
- glucose + fructose-> sucrose
- glucose + galactose-> lactose
What happens when monosaccharides join together
Condensation reaction - a molecule of water is removed
a glycosidic bond is formed
Test for non- reducing sugars
1) Grind up sample into liquid form
2) Add 2cm3 of food sample to be tested and an equal volume of blue Benedict’s reagent(copper II sulphate) into a test tube
3) Filter the mixture
4) Heat the mixture in a gently boiling water bath for 5 minutes. If the solution doesn’t change colour, then a reducing sugar isn’t present
5) Add another 2cm3 of food sample to2cm3 of dilute hydrochloric acid in a test tube in a gently boiling water bath for 5mins. The dilute HCl will hydrolyse any disaccharide present into its constituent monosaccharides
6) Slowly add some sodium hydrogen carbonate solution to the test tube in order to neutralise the HCl(Benedict’s reagent won’t work in acidic conditions). Test with pH paper to check solution is alkaline
7) Re-test solution by heating it with 2cm3 of Benedict’s reagent in gently boiling water bath for 5mins
8) If non-reducing sugar is present in original sample, solution will now turn orange-brown.
Example of non-reducing sugar
sucrose
Polysaccharide
polymers formed from many repeating monosaccharide sub-units
joined by glycosidic bonds formed from condensation reaction
very large molecule so is insoluble- suitable for storage