1. Laboratory Glassware Flashcards
What does precision or reproducibility refer to?
The ability to repeatedly produce the same value
What does capacity refer to?
The volume which the glassware will hold or deliver.
Is temperature marked on glassware? What does it refer to?
Usually marked at 20 degrees.
Refers to the temperature at which the glassware was calibrated.
What does TC stand for? Give examples of some TC glassware.
TC = to contain
Glassware has been calibrated to hold the stated volume, and deliver less than the stated volume
E.g. Volumetric flasks, graduated cylinders
What does TD stand for? Give some examples of TD containers.
TD = to deliver
The exact volume will be delivered when a liquid is being drained as the glassware was calibrated to account for the liquid adhering to the walls.
E.g. Pipette, some graduated cylinders
What is a meniscus?
Caused by surface tension that draws up the edges of a liquid.
Lowest part of the meniscus should be directly on the calibration mark.
How should test tubes be carried?
Always in a rack
Where should pipettes be held?
Always in a rack
What should you do when you make any control or reagent?
Label with year, month, date and initial
What are the conversions for mL?
1L = 1000mL 1mL = 1000 microL
What does a volumetric pipette look like?
Glass, with a bulb in the middle, calibrated to hold mL, is calibrated to the tip.
What does a Mohr pipette look like?
Glass pipette no bulge, measured in mL, NOT calibrated to the tip.
What are the semiautomatic pipettes?
MLA, Eppendorf, Hamilton
What is a Pasteur pipette?
Can be glass or plastic, disposable, small, used to transfer liquid from one container to another
What do Erlenmeyer flasks and volumetric flasks look like?
Erlenmeyer - triangular in shape
Volumetric - skinny at top, bulb at bottom
