1-enzymes

Question 1

What is in the active site of enzyme?

Answer 1

A small section (2-4 amino acids which bind to substate molecule)
Thus the substrate is complementary to enzyme.

Question 2

Explain the lock and key method

Answer 2

Substrate is key which fits in the lock which is the enzyme forming a temporary structure which is the enzyme-substrate complex and releases it as products

Question 3

Explain the induced fit model

Answer 3

The concept which the enzyme active site changes shape to fit substrate and it form and enzyme substrate complex and break down the substrate and it now product complex and releases it as products

Question 4

Define a catalyst

Answer 4

A catalyst is a chemical which speeds up the rate of reaction by lowering its activation energy
Without being used up

Question 5

Explain why enzymes are effective in small quantities

Answer 5

They are not used up in reaction so they can be reused

Question 6

Outline why changing one amino acids that make up active site could prevent the enzyme functioning

Answer 6

The substrate will no longer bind to enzyme which will then not be positioned correctly if at all in the site

Question 7

Explain why changing certain amino acids that are not part of active site prevents enzymes from functioning

Answer 7

The changed amino acid may be the one hunch form hydrogen bond with other amino acids
The tertiary structure of enzymes changes if the new amino acid don’t form hydrogen bond including active site so now substrate can’t fit in enzyme

Question 8

Compare and contrast the inducted fit model to lock and key model

Answer 8

-E-P-C in induced fit model whilst L-A-K don’t have it
-enzyme has to go through confirmation in his active site to fit substrate

-both form a E-S-complex
-Both involve substrate and enzyme reacting
-both release the substrate as products
-both go through transition site