1: ENTAMOEBA HISTOLYTICA Flashcards

1
Q

Common associated disease or condition names

A

Intestinal amebiasis, amebic colitis, amebic dysentery, extraintestinal amebiasis

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2
Q

e trophozoite exhibits rapid, unidirectional,
progressive movement, achieved with the help of ______________________

A

finger-like hyaline pseudopods

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3
Q

The single nucleus typically contains a small central mass of chromatin known as _________

A

karyosome (karyosomal chromatin)

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4
Q

The karyosome of this amebic parasite is surrounded by chromatin material, a morphologic structure called ___________ ____________.

A

peripheral chromatin

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5
Q

Motility

A

Progressive, finger-like
pseudopodia

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6
Q

Number of nuclei

A

One

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7
Q

Karyosome

A

Small and central

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8
Q

Peripheral chromatin

A

Fine and evenly distributed

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9
Q

Cytoplasm

A

Finely granular

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10
Q

Cytoplasmic inclusions

A

Ingested red blood cells

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11
Q

The E. histolytica trophozoite contains a ____________ ____________ __________, which is often referred to as having a ground glass in appearance.

A

finely granular cytoplasm

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12
Q

Young cysts characteristically contain unorganized chromatin material that transforms into squared or round-ended structures called _____________

A

chromatoid bars

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13
Q

structures that contain condensed RNA material.

A

chromatoid bars

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14
Q

It is a cytoplasmic area without defined borders that is believed to represent stored food, is also usually visible in young cysts

A

glycogen mass

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15
Q

Nuclear variations during maturation

A
  • eccentric (rather than the typical central) karyosomes
  • thin plaques of peripheral chromatin, or
  • a clump of peripheral chromatin at one side of the nucleus that appears crescent shaped.
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16
Q

The mature infective cyst is

A

quadrinucleated

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17
Q

RBCs, bacteria, yeast, and other debris are not found in the cyst stage.

A

True

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18
Q

Laboratory Diagnosis

A
  • traditional wet preparation
  • permanent staining techniques
  • sigmoidoscopy procedure
  • hepatic abscess material
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19
Q

A special medium known at ________ supports E. histolytica in culture.

20
Q

Once the infective cyst is ingested, excystation occurs in the __________________.

A

small intestine

21
Q

As a result of the nuclear division, a single cyst produces _____ motile trophozoites.

22
Q

These motile amebas settle
in the ______ of the large intestine, where they replicate by ________ fission and feed on living host cells

A

lumen, binary

23
Q

Encystation occurs in the _________________

A

intestinal lumen

24
Q

cyst formation is complete when ___ nuclei are present.

25
Liquid or semi-formed samples may show __________ if the intestinal motility is rapid.
trophozoites
26
_________ will form, on the other hand, if the intestinal motility is normal.
Cysts
27
The range of symptoms varies and depends on two major factors:
(1) the location(s) of the parasite in the host; and (2) the extent of tissue invasion
28
Asymptomatic Carrier State
(1) the parasite is a low-virulence strain; (2) the inoculation into the host is low; and (3) the patient’s immune system is intact.
29
Symptomatic Intestinal Amebiasis
- amebic colitis - amebic dysentery (characterized by blood and mucus in the stool)
30
Symptomatic Extraintestinal Amebiasis.
- trophozoites that migrate into the bloodstream are removed by and take up residence in the liver. - amebic pneumonitis - Venereal amebiasis - Penile amebiasis
31
Treatment
Asymptomatic individuals may be treated with: - paromomycin - diloxanide furoate (Furamide) - metronidazole (Flagyl) Patients showing symptomatic intestinal amebiasis: - iodoquinol paromomycin diloxanide furoate Symptomatic intestinal amebiasis treatment: - Metronidazole - Tinidazole
32
Entamoeba except E. histolytica, E. dispar, and E. moshkovskii are differentiated by
isoenzyme analysis polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and typing with monoclonal antibodies
33
most invasive of the Entamoeba parasites and the only member of the family to cause colitis and liver abscess
Entamoeba histolytica
34
The quadrinucleate cyst is resistant to ____________ and __________
gastric acidity, desiccation
35
Trophozoites have the ability to colonize and/or invade the ___________, while cysts are never found within invaded ________.
large bowel, tissues
36
E. histolytica trophozoites multiply by ___________.
binary fission
37
They encyst producing uninucleate cysts, which then undergo two successive nuclear divisions to form the characteristic ______________________
quadrinucleate cysts
38
nuclear-encoded mitochondrial genes
pyridine nucleotide transhydrogenase and hsp60
39
most common extra-intestinal form of amebiasis.
Amebic liver abscess (ALA)
40
cardinal manifestations of ALA
fever and right upper quadrant (RUQ) pain
41
The most serious complication of amebic colitis is
perforation and secondary bacterial peritonitis
42
activated macrophages produce _______________ which was lethal to trophozoites.
nitric oxide (NO)
43
The standard method of parasitologic diagnosis is microscopic__________________________.
detection of the trophozoites and cysts in stool specimens
44
Stool culture using _____________________is more sensitive than stool microscopy, but is not routinely available.
Robinson’s and Inoki medium
45
gold standard
ELISA & PCR
46
____________ is the drug of choice for the treatment of invasive amebiasis
Metronidazole
47
____________________ of liver abscess is indicated for patients who do not respond to metronidazole and who need prompt symptomatic relief of severe pain
Percutaneous drainage