1. Cell Biology Flashcards
What are eukaryotic cells?
More complex, eg. animal or plant cells.
What are eukaryotes?
Organisms made up of eukaryotic cells.
What are prokaryotic cells?
Smaller and simpler, eg. bacteria.
What is a prokaryote?
A prokaryotic cell (single-celled organism)
What is the function of the nucleus?
Contains genetic material that controls the activities of the cell.
What is the cytoplasm?
Gel-like substance where most chemical reactions happen. It contains enzymes that control these chemical reactions.
What is the function of the cell membrane?
Holds the cell together and controls what goes in and out.
What is the function of the mitochondria?
Where most of the reactions for aerobic respiration takes place. Respiration transfers energy that the cell needs to work.
What are ribosomes?
Where proteins are made in the cell (protein-synthesis).
What do plant cells contain that animal cells don’t?
Cell wall, permanent vacuole and chloroplasts.
What is the function of the rigid cell wall?
Made of cellulose. It supports the cell and strengthens it.
What is the function of the permanent vacuole?
Contains cell sap, a weak solution of sugars and salts.
What are chloroplasts?
Where photosynthesis occurs, which makes food for the plant. They contain chlorophyll (green substance), which absorbs the light needed for photosynthesis.
What are the sub-cellular structures of a bacteria cell?
Cytoplasm, cell membrane, cell wall, circular strand of DNA, plasmids.
What don’t bacteria cells contain?
Chloroplasts and mitochondria.
What do bacteria cells have instead of a nucleus?
Single circular strand of DNA floating freely in the cytoplasm and small rings of DNA called plasmids.
What are light microscopes?
Use light and lenses to form an image of a specimen and magnify it. They let us see individual cells and large subcellular structures like nuclei.
What are electron microscopes?
Use electrons to form an image. Have a much higher magnification than light microscopes. Also have a higher resolution. Let us see much smaller things in more detail, like internal structures of mitochondria and chloroplasts. Can even see ribosomes and plasmids.
What is resolution?
The ability to distinguish between two points, so higher resolution gives a sharper image.
What is the equation for magnification?
magnification= image size/real size.
Describe how you would carry out the microscopy practical to view and onion cell?
- Add a drop of water to the middle of a clean slide.
- Cut up and onion and separate it out into layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers.
- Place the epidermal tissue into the water on the slide.
- Add a drop of iodine solution as a stain. -used to highlight objects in a cell by adding colour to them.
- Place a coverslip on top (carefully tilt lower so it covers the specimen). Try not to get air bubbles as this will obstruct the view of the specimen.
How do you use a light microscope?
- Clip the slide you’ve prepared onto the stage.
- Select the lowest-powered objective lens (lowest magnification).
- Use the coarse adjustment knob to move the stage up to just below the objective lens.
- Look down the eyepiece. Use the coarse adjustment knob to move the stage downwards until the image is roughly in focus.
- Adjust the focus with the fine adjustment knob, until you get a clear image of what’s on the slide.
- If you need to see the slide with greater magnification, swap to a higher-powered objective lens and refocus.
What is differentiation?
The process by which a cell changes to become specialised for its job.
When does most differentiation occur in an animal cell compared to plant cells?
The ability to differentiate is lost at an early stage, after they become specialised. However, lots of plant cells don’t loose this ability.