1. Biological Molecules Flashcards
Describe how calibration curve is carried out:
-reducing tests of known glucose concentrations carried out (6 different concentration)
-colorimeter used on all of them to quantify amount of glucose
-reducing test done on unknown concentration solution
-colorimeter used to quantify by comparing results of unknown to known solutions to predict concentration of unknown
Two molecules of glucose can react during a ……. ……. to form a ……. …… and lose a water molecule, forming a disaccharide. This forms a …. …..
Two molecules of glucose can react during a condensation reaction to form a glycosidic bond and lose a water molecule, forming a disaccharide.
This forms a 1-4 bond.
A primary structure is ……. ….. (3)
- specific sequence of amino acids
- joined by peptide bonds
- by cond. reaction
A secondary structure is
-folding of the polypeptide chain due to h-bonds betw/ NH and C=O ➜ folds into alpha helix / beta pleated sheet
Tertiary structure:
- further folding into 3D shape betw/ multiple amino acids joined by pep bonds
- due to bonds forming betw/ diff R groups in amino chain (h-bonds, ionic, disulfide)
quaternary structure:
- folding & bonding betw/ >=2 polypep chains
- forms fibrous/ globular structures
cellulose is a polymer of
beta glucose
Define reducing sugars:
Reducing sugars can donate an electron to another molecule, eg:
-all monosaccharides
-maltose, lactose
describe how a triglyceride is formed
- one molecule of glycerol and 3 fatty acids
- condensation reaction, 3 molecules of water removed
forms ester bonds
glycogen is made w
alpha glucose
describe test for lipid
- add ethanol
- add water
- shake
- white emulsion
Describe the structure of starch and explain how it’s adapted for its function in cells: (6 marks)
- coiled in a tight helix
➔makes it compact and allows for maximum glucose storage to be used as energy during respiration - Insoluble in water
➔doesn’t affect water potential so doesn’t cause water to move into the cells through osmosis - Large polymers
➔can’t diffuse out of the cell membrane - has a lot of ends from a lot of branches
➔many ends for enzymes to act on during hydrolysis, starch is broken down rapidly
Explain one other effect on the plant caused by the evaporation of water from its leaves
- Cools leaves
- Evaporation uses heat from leaves
Explain why a change in primary structure can affect the structure of a protein: (4)
- means diff amino acids in the sequence
- different R groups in different places
- changes in bonding in tertiary structure between R groups (ionic, h-bonds, disulfide)
- protein will fold into a different shape and function will change
Explain why this column of water doesn’t break (3 marks)
Water molecules polar
Form hydrogen bonds betw water molecules
Cohesion prevents breakage of columns
Give properties of water that are important in Biology (5)
- Polar molecules: Acts as an universal solvent
- Metabolite: Involved in metabolic reactions such as condensation and hydrolysis
- Water molecules stick together, cohesive bonds between water molecules: Provides surface tension/ prevents columns of water breaking
- High specific heat capacity: Reduces fluctuations in temperature (of water bodies)
- High latent heat of evaporation: Evaporation of small amount of water cools an organism
how is cellulose adapted for function in plant cells
- straight and long chains
- many hydrogen bonds between chains form fibrils
- makes cell wall very strong, resistant to osmotic pressure
how is starch adapted for function in plant cells
- Insoluble;
- Don’t affect water potential;
- Helical;
- Compact;
- Large molecule;
- Cannot leave cell.
If there is a reducing sugar present then:
The Cu2+ from the Benedicts solution will gain an electron and form C+ ions.
- These form a red precipitate.
- If concentration of reducing sugars is low then: less precipitate, more Cu2+ ions left in the solution (less transmission, more absorption)
- Solution will be bluer
lactose formed by cond. of
glucose and galactose
2 major stores of glycogen in humans
liver and muscle cells
Test for non-reducing sugars: (sucrose)
- take boiling tube, add dilute HCl (to hydrolise)
- add Sodium hydrogen carbonate (to neutralise it)
(pH should be 7) - add Ben’s solution
- heat by putting boiling tube in beaker w/ boiling water
observe color change
hydrolysis reaction to reverse a peptide bond is carried out by
protease enzymes in digestive system
what is a monomer
small repeating unit from which a larger molecule is made