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Question 1

If we use separating solution for isolation of mononuclear cells, we need -

Answer 1

Gently overlay separating solution with blood and then centrifuge the samples (2500 rpm/15 min)

Question 2

Phagocytosis is property of -

Answer 2

Neutrophils and monocytes/macrophages

Question 3

Which of the following components is for IFA -

Answer 3

Antibody labelled with fluorochrome

Question 4

The direct IFA is used for -

Answer 4

Determination of antigen in a cell or tissue by direct labelling with antibodies labelled with FITC

Question 5

The indirect IFA is most commonly used for -

Answer 5

Testing of autoimmune disorders

Question 6

To develop a test to screen circulating anti-brucella antibodies by immunofluorescence, you need all of the following EXCEPT -

Answer 6

Isolated patient’s IgG labelled with a fluorescein compound

Question 7

FACS is used for -

Answer 7

Analysis and sort a mixture of the cells based on their differential ability to bind fluorescence-dye labelled ATB

Question 8

The essential step for western blotting diagnosis of BSE is -

Answer 8

Treatment of the sample with proteinase K

Question 9

Serological reaction is

Answer 9

Serology is the scientific study of blood serum and other body fluids Serological reaction is Ag + Ab → AgAb, antigen binds to the antibody

Question 10

The titer of an antibody is:

Answer 10

The highest dilution of an antiserum that still react with antigen in serological reactions (X)

Question 11

Serum is obtained after:

Answer 11

Centrifugation of clotted blood

Question 12

Visible clumping - agglutination. Agglutination is used to determine presence of antibodies/antigens Agglutination reaction is a reaction between:

Answer 12

Corpuscular Ag and polyclonal Ab