1

Question 1

ELF4E FUnction (2 things)

Answer 1

Binds to 5’ Cap

Affected by mTOR pathway

Question 2

ElF2 (2)

Answer 2

Binds to and delivers starting Met-tRNA
Uses GTP
Release with GTP dephosphorylation indicates big subunit should bind

Question 3

When is elf2 phosphorylated

Answer 3

Low AA, Cell stress, Immune response, unfolded proteins

Phosphorylated is turn off

Question 4

EFL4G (2)

Answer 4

Scaffold

Binds to ELF4E

Question 5

initiator tRNA-when used

Answer 5

Not used during elongation

Question 6

GTP hydolysis (3)

Answer 6

ELF2 release, EEF2-ratchet down, EEF1A-drops off tRNA to A site

Question 7

EEF1A

Answer 7

Binds to tRNA with AA on it
Has GTP
When loses GTP drops off tRNA to A site

Question 8

EEF2

Answer 8

Pushes whatever in A site to E site
Has GTP
When to GDP ratchets down

Question 9

When GTP regulation use

Answer 9

EIF2, EEF1A, EEF2

Question 10

ERF1

Answer 10

Terminator tRNA-catalyzes finished peptide bond from ribosome

Question 11

Shine Delgarno

Answer 11

Prok enhancer-binds to ribo RNA

Question 12

Selective inhibition

Answer 12

Prok ribosomes small and divergent from mamlalian

-can inhibit them and not ours

Question 13

mono vs polycistronic

Answer 13

Proks are poly

Many proteins made from 1 mRNA

Question 14

miRNA

Answer 14

Creates RISC sequences- no room for initiation

Question 15

ApoB

Answer 15

Long in liver
Short in intestine
Premature stop codon makes smaller

Question 16

IRE

Answer 16

Iron recognition element

-translation of mRNA regulated by iron bonding

Question 17

ElF2B

Answer 17

Makes GDP go back to GTP on ELF2

• poor conditions-ELF2 gets phosphorylated and binds to ELF2B
  
  • sequesters ELF2B
  • No GDP to GTP

Question 18

mTOR pathway

Answer 18

rheostat of cell

• effects ELF4E-repressess trans
• activated during growth
• Causes 4EBP phosphorylation and increased trans

Question 19

mTOR pathway and hypoxia

Answer 19

inhibits mTOR

-allows 4EBP dephosphorylation and binds to ElF4E, causing decreased translation

Question 20

4EBP

Answer 20

Binds to elF4e
inhibits trans
When phosphorylated, released from elF4e and translation continues

Question 21

Phosphorylation reguation

Answer 21

EF2B and 4EBP

Question 22

DIO

Answer 22

deodinases required for TH synthesis

-made from selenium

Question 23

eFSEC

Answer 23

specialized elongation factor for Sec-AA tRNA

Question 24

Sec-tRNA recognizes

Answer 24

UGA

Question 25

SECIS element

Answer 25

RNA in 3’ UTR that allows SECIS incorporation in translated region

Question 26

SBP2

Answer 26

Secis binding protein 2
Binds to SECIS element and assists in getting EFSec to ribosome at UGA codons near SECIS element
Mutations result in thyroid cancer

Question 27

Poliovirus

• Pathway
• Novel ability

Answer 27

Expressed protease that cleaves ElF4G
Poliovirus IREs bind to cleaved ElF4G and recuirt pre-initation complex directly (no need for cap)

Permits efficient viral mRNA translation and hurts host translation

Question 28

Diptheria

Answer 28

eEF2 inactivation

no trans