1 Flashcards
Made up of a series of lenses and utilizing visible light as its source of illumination, the
________ microscope can magnify an object 1,000 to 1,500 times
Brightfield Microscope
This type of microscopy
was first introduced by Frits Zernike
Phase-contrast Microscope
It was developed by Georges Nomarski in 1952 as an improvement to the phase contrast
microscope.
Differential Interference Contrast Microscope
was developed in the 1980s by the Swiss scientists Dr. Gerd
Binnig and Dr. Heinrich Rohrer. It is used to study the molecular and atomic shapes of organisms
on a nanoscale
Scanning Probe Microscope
is an aqueous or alcohol solution of a single basic dye
simple stain
also known as the “hot method” because it requires steam
bathing the prepared smear after addition of the primary dye
Ziehl-Neelsen stain
also known as the “cold method”
. Kinyoun stain
is a means of
determining the organism’s virulence
Negative Staining for Capsules
The most commonly used endospore stain is the
Schaeffer-Fulton endospore stain.
A tedious and delicate staining
procedure uses a mordant and the stain carbolfuchsin to build up the diameters of the flagella
until they become visible under the light microscope
Flagella Staining