#1 Flashcards
What is the function of the ocular/eyepiece in a microscope?
Contains a lens normally with a 10 x magnification with pointer to aid in locating objects within the field of view.
What is the role of the arm handle in a microscope?
Attachment for other mechanical parts.
What does the revolving nosepiece do?
Holds and shifts the objectives.
What are the different types of objectives in a microscope?
- Scanner: 4x
- Low Power Objective: 10x
- High Power Objective: 40x
- Oil Immersion Objective: 100x
What is the purpose of the stage in a microscope?
Platform on which slide is positioned.
What do stage clips do?
Hold the slide in place and move the slide on the stage.
What is the function of the iris diaphragm?
Controls the amount of light.
What does the condenser do in a microscope?
Gathers light from the microscope’s light source and concentrates it into a cone of light that illuminates the specimen.
What is the light source/illuminator in a microscope?
Reflects light through the specimen.
What is the coarse adjustment knob used for?
Appropriate for focusing on Scanner or Low Power Objective.
What does the fine adjustment knob do?
For final focusing.
What is the base of a microscope?
Serves as a support for microscopes.
Fill in the blank: The stage micrometer and ocular micrometer are used for _______.
[Calculating Calibration]
What are the magnifications associated with different objectives?
- 4x (Scanner) = 25
- 10x (Low Power Objective) = 10
- 40x (High Power Objective) = 2.5
- 100x (Oil Immersion Objective) = 1
What are the characteristics of a compound microscope compared to a dissecting microscope?
- Compound Microscope: 4x, 10x, 40x, 100x (Lower), 2D, Inverted, Opposite of movement being done
- Dissecting Microscope: 5x, 50x (Higher), 3D, Upright, Aligned with movement being done
What are the three visible parts of an animal cell under a light microscope?
- Cell Membrane
- Nucleus
- Cytoplasm
What is the function of the cell membrane?
Surrounds the cell and regulates the flow of substances between the cell and its surroundings.
What is the nucleus of a cell?
Usually spherical or ovoid structure that contains the genetic material.
What is cytoplasm?
The substance outside the nucleus where the other structures such as organelles are located.
What stain is used to view cellular structures in human cheek cells?
Methylene blue.
What is the shape of frog stomach cells?
Fusiform/spindle.