1 Flashcards
Structure and function starch
Alpha glucose
Amylose 1-4 gyclosidic bonds
Amylopectin 1-4,1-6 glycosidic bonds
Insoluable - doesn’t affect water potential
Large molecule can’t leave cell
Highly branched - large SA
Hellical so compact
Structure and function of glycogen
Alpha glucose
1-4,1-6 glycosidic bond
Insoluble
Highly branched
Unsaturated fatty acid
Has a double C=C bond between carbon atoms in hydrocarbon chain
Properties triglycerides
- Doesn’t affect water potential- large and hydrophobic so insol
- Low mass - lot can be stored without increasing mass
- High ratio oxygen hydrogen atoms = metabolic water source
Temperature effect enzyme
To low = not enough kenetic energy
To high = denature enzymes
Ph effect enzymes
Break ionic hydrogen bonds
Change tertiary structure
Active site diff
Semi conservative rep
- Strands seperate
- Dna helicase unwinds by breaking h bonds
- Both stands = template
- Free nucleotides attach
- Comp bases A-T C-G
6 DNA polymerase joins adjacent nucleotides form phosphodiester bonds
ATP key properties
- Phosphorlate compounds
- Resleased small packets prevent loss
- Released instantaneously
- Easily resynthasied
Optical vs electron
Light beam vs electron
Poor resolution vs high
Low magnification vs high
Colour vs black and white
Living vs non living
TEM vs SEM
2D vs 3D
Higher resolution vs lower
Thin specimens vs not thin
Cell fractionation
- cell broken down seperate organelles
- using blender
- homogenisation cells kept in cold (reduce enzyme activity) isotonic (water potential no bursting) buffer (ph) solution
- filter to remove large debris
Ultracentrifugation
- solution in cebtrifuge spins at high speed separate organelles on density
- NCMLER
Transcription
- Hydrogen bonds break
- One stand = template
- Rna nucleotides align next to comp base
- C-G U-T
- Rna polymerase joins nucleotides
- Phosphodiester bonds
- Splice introns (eukaryotic)
Translation
- Mrna bind ribosome
- Anticodon trna comp codon on mrna bind
- Trna bring specific amino acid
- Amino acid join by peptide bonds
- Using atp
- Trna released
- Ribosome moves along mrna to form polypeptide
Two diff between mitosis and meiosis
One division vs two
Identical cell vs not identical
No crossing over vs is
No independent seg vs is