protein structure and function - specification

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Decks in this class (16)

OC1 - general principles of denaturation
What is denaturation,
Primary protein structure,
Secondary protein structure
12  cards
OC1 - inactivation of functional sites
Preservation of cysteine residues
1  cards
OC1 - prevention of proteolysis
Proteolysis,
Protease inhibitor cocktail
2  cards
OC1 - protein storage
Methods of storage,
Low temperature storage,
Cryoprotectant
3  cards
OC2 - general characteristics of binding sites
Binding site,
Chemical specificity,
Affinity
10  cards
OC2 - interactions involved in protein-ligand binding
Chemical bonding at the binding site
1  cards
OC2 - structural methods for locating the amino acid residues in binding sites
Requirements for x ray crystallog...,
Process of x ray crystallography,
Structural analogues
4  cards
OC2 - chemical methods for locating the amino acid residues in binding sites
Chemical modification,
Chymotrypsin,
Ph dependence of activity
3  cards
OC3 - role of conformational changes
Allosteric enzymes,
Modulators,
Positive modulator
5  cards
OC3 - phosphorylation and dephosphorylation
Covalent modification,
Phosphorylation,
Protein kinases
5  cards
OC3 - activation by proteolysis
Proteolytic activation,
Pepsinogen
2  cards
OC3 - regulation by binding proteins
Signal transduction,
Adenylate cyclase system
2  cards
OC4 - proteomics
Definition of proteomics,
Aim of proteomics
2  cards
OC4 - sequencing and identifying proteins
How are proteins sequenced and id...,
Sample preparation,
Separation
9  cards
OC4 - determination of chemical bonding at the binding site
Antibody trapping,
Fret fluorescence resonance energ...,
Yeast 2 hybrid assay
4  cards
exam questions and answers
Define denaturation,
Describe the bonds involved in th...,
During denaturation which level o...
55  cards

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protein structure and function - specification

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