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Adam Bills
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Decks in this class (22)
L1 Antibody structure & function
Describe general antibody structure and understand how this relates to function.
Distinguish structural features of antibodies that are related to antigenicity.
Understand and explain clonal selection and the generation of antibody diversity.
Compare and contrast all antibody class structure/functions.
30
cards
L2 Introduction to antibody-based assays
Understand the antibody-antigen interaction.
Understand the importance of antibodies in biomedical research.
Understand and explain various uses and limitations of antibodies.
Describe general principles of antibody based assays.
23
cards
L3 Toxicity testing in cultured cell systems
Discuss the principles of cell culture, the main types of cultures cells, and the benefits and drawbacks of using this approach in toxicology.
Understand the basic principles of cell culture.
Explain how cultured cells are produced and maintained;
critically evaluate their use in toxicity studies.
18
cards
L4 Introduction to end point measurements for toxicity testing in cultured cell systems
To introduce the general principles of end point determination in toxicity testing and to discuss some of the key approaches used to study cell viability, proliferation and metabolic activity.
Understanding of the basic requirements of end point measurements.
Ability to discuss key methods used to monitor cell viability, proliferation and metabolic activity.
Ability to explain how to combine them effectively in a tiered strategy of toxicity testing.
9
cards
L5 Non-competitive immunoassays
Critically discuss the basis of immunoblotting.
Critically discuss the use of immunocytochemical/histochemical techniques.
Explain and understand how immunoassays may be applied in molecular bioscience and biomedical research.
32
cards
L6 Molecular end points in toxicity testing in vitro
Aims: To introduce the general principles of molecular end point determination in toxicity testing, discuss some of the key approaches used and their application in a tiered screening strategy.
Outcomes:
- Understanding of the basic principles of molecular end point measurements
- Ability to discuss key molecular methods and how to combine them in an appropriate manner in a tiered strategy of toxicity testing
9
cards
L7 General and specific uses of mAbs
- Understand how the characteristics of mono and poly antibodies influence how they are used.
- Discuss the applications of rodent mono antibodies
- Review 'on site' assays
- Know how monoclonal antibodies are used in immunoaffinity purification, as research tools and as anti-toxins.
- Know how mono antibodies are currently used therapeutically, and appreciate their future potential.
25
cards
L8 The uses of antibodies in the study of cell signalling
What are six main uses of antibod...,
Besides the main six uses of anti...,
What are the two steps of western...
26
cards
CCAT L9 Polyclonal Antibodies
Lecture outline:
Define the main steps involved in generating polyclonal antibodies, the problems involved at various stages and how they are overcome.
Suggest why anti-peptide antibodies can be useful and how they are produced.
17
cards
L10 Monoclonal Antibody Production
Learning outcomes:
Explain how murine hybridomas are produced and understand the problems that may arise.
Understand the differences between conventional serum and monoclonal antibody derived from a hybridoma.
Learn about industrial production of mAbs
23
cards
L11 Role of phage display in antibody production
Explain steps involved in phage display of antibody fragments.
Understand link between phenotype and genotype.
Importance of this procedure for human antibody production.
Limitations of this method.
21
cards
L12 Making humanised mAbs
Learning outcomes:
To explain why rodent antibodies are limited in human therapy.
To explain somatic recombination.
Understand how rodent antibodies are made more human.
Outline how human antibodies can be produced in transgenic animals
15
cards
L13 Uses of mAbs - therapeutics
Understand how the characteristics of monoclonal and polyclonal antibodies influence how they are used.
Discuss the applications of rodent monoclonal antibodies.
Review 'on site' assays.
Know how monoclonals are used in immunoaffinity purification, as research tools and as anti-toxins.
Know how monoclonal antibodies are currently used therapeutically, and appreciate their future potential.
14
cards
L14 Cellular models of neurotoxicity
Aims:
To discuss examples of cellular models of neurotoxicity due to oxygen deprivation, glutamate and metamphetamine exposure.
Outcomes:
Familiar with several examples of cellular models
Develop the ability to design a cellular model for the study of specific neurotoxins
10
cards
L15 Cellular Models of Neurotoxicity pt2
What toxin is used commonly in ne...,
How does op elicit acute toxicity,
What are the known changes that o...
7
cards
L16 The use of antibodies in the study of cell signalling pt2
How can proteome be studied in ce...,
Which three amino acids are phosp...,
Give examples of kinases that pho...
18
cards
L17 Cellular Models of Diabetes
What is the key organ involved in...,
What pancreatic cells have been c...,
What three other tissues cells ar...
18
cards
L18 Stem Cell Culture in Neurotoxicity
Aims:
To discuss the sources of stem cells and their validity for studies of neural development and developmental neurotoxicity.
Outcomes:
To be able to evaluate different stem cell models in use or under development and to evaluate their application in neurotoxicity studies.
11
cards
L19 Oxidative stress pt 1
- Investigate oxidative stress using cellular models.
LOs:
- Explain the biochemistry of reactive oxygen species, their origin and the cellular mechanisms of protection against these agents.
- Identify some of the methods that can be used to measure ROS and its consequences in cell models.
- Identify the links between ROS, irregular biochemical processes and disease.
20
cards
L20 Antibodies in cell signalling pt 3
Protein phosphorylation
9
cards
L21 Antibodies in cell signalling pt 4
How can the phospho protein conte...,
What is a phospho protein array,
What does flow cytometry allow in...
9
cards
L22 Oxidative stress pt 2
When does hydrogen peroxide becom...,
What is a key defense against fre...,
What is the role of the glutathio...
23
cards
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Cell culture & antibody technology
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